RRC ID 42406
著者 Ban S, Michikawa Y, Ishikawa K, Sagara M, Watanabe K, Shimada Y, Inazawa J, Imai T.
タイトル Radiation sensitivities of 31 human oesophageal squamous cell carcinoma cell lines.
ジャーナル Int J Exp Pathol
Abstract The purpose of this study was to determine the radiosensitivities of 31 human oesophageal squamous cell carcinoma cell lines with a colony-formation assay. A large variation in radiosensitivity existed among 31 cell lines. Such a large variation may partly explain the poor result of radiotherapy for this cancer. One cell line (KYSE190) demonstrated an unusual radiosensitivity. Ataxia-telangiectasia-mutated (ATM) gene in these cells had five missense mutations, and ATM protein was truncated or degraded. Inability to phosphorylate Chk2 in the irradiated KYSE190 cells suggests that the ATM protein in these cells had lost its function. The dysfunctional ATM protein may be a main cause of unusual radiosensitivity of KYSE190 cells. Because the donor of these cells was not diagnosed with ataxia telangiectasia, mutations in ATM gene might have occurred during the initiation and progression of cancer. Radiosensitive cancer developed in non-hereditary diseased patients must be a good target for radiotherapy.
巻・号 86(4)
ページ 231-40
公開日 2005-8-1
DOI 10.1111/j.0959-9673.2005.00431.x
PII IEP431
PMID 16045545
PMC PMC2517430
MeSH Ataxia Telangiectasia Mutated Proteins Blotting, Western / methods Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / radiotherapy* Cell Cycle Proteins / genetics Cell Line, Tumor DNA Repair / genetics DNA, Neoplasm / genetics DNA-Activated Protein Kinase DNA-Binding Proteins / genetics Esophageal Neoplasms / genetics Esophageal Neoplasms / radiotherapy* Humans Immunohistochemistry / methods Mutation, Missense / genetics Neoplasm Proteins / genetics Nuclear Proteins Phosphorylation Protein Serine-Threonine Kinases / genetics Radiation Tolerance / genetics Tumor Suppressor Proteins / genetics
IF 1.672
引用数 8
WOS 分野 PATHOLOGY
リソース情報
ヒト・動物細胞