Reference - Detail
| RRC ID | 42416 |
|---|---|
| Author | Okumura S, Saitoh H, Ishikawa T, Wasano N, Yamashita S, Kusumoto K, Akao T, Mizuki E, Ohba M, Inouye K. |
| Title | Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines. |
| Journal | J Agric Food Chem |
| Abstract |
Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL. |
| Volume | 53(16) |
| Pages | 6313-8 |
| Published | 2005-8-10 |
| DOI | 10.1021/jf0506129 |
| PMID | 16076112 |
| MeSH | Animals Antineoplastic Agents / pharmacology* Bacillus thuringiensis / chemistry* Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Death / drug effects Cell Line Cell Line, Tumor Cells, Cultured Escherichia coli / genetics Gene Expression Humans Recombinant Proteins / pharmacology |
| IF | 4.192 |
| Times Cited | 25 |
| WOS Category | AGRICULTURE, MULTIDISCIPLINARY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | MOLT-4(RCB0206) Jurkat(RCB0806) HL60(RCB0041) Sawano(RCB1152) Hep G2 HeLa(RCB0007) TCS(RCB0638) A549(RCB0098) MRC-5 CACO-2(RCB0988) COS-7(RCB0539) K562(RCB0027) CHO-K1(RCB0285) PC-12(RCB009) U-937 DE-4(RCB0435) Vero(RCB0001) NIH3T3 |