RRC ID 4243
Author Hüsken K, Wiesenfahrt T, Abraham C, Windoffer R, Bossinger O, Leube RE.
Title Maintenance of the intestinal tube in Caenorhabditis elegans: the role of the intermediate filament protein IFC-2.
Journal Differentiation
Abstract The Caenorhabditis elegans intestinal lumen is surrounded by a dense cytoplasmic network that is laterally attached to the junctional complex and is referred to as the endotube. It localizes to the terminal web region which anchors the microvillar actin filament bundles and is particularly rich in intermediate filaments. To examine their role in intestinal morphogenesis and function, C. elegans reporter strains were generated expressing intestine-specific CFP-tagged intermediate filament polypeptide IFB-2. When these animals were treated with dsRNA against intestinal intermediate filament polypeptide IFC-2, the endotube developed multiple bubble-shaped invaginations that protruded into the enterocytic cytoplasm. The irregularly widened lumen remained surrounded by a continuous IFB-2::CFP-labeled layer. Comparable but somewhat mitigated phenotypic changes were also noted in wild-type N2 worms treated with ifc-2 (RNAi). Junctional complexes were ultrastructurally and functionally normal and the apical domain of intestinal cells was also not altered. These observations demonstrate that IFC-2 is important for structural maintenance of the intestinal tube but is not needed for establishment of the endotube and epithelial cell polarity.
Volume 76(8)
Pages 881-96
Published 2008-10
DOI 10.1111/j.1432-0436.2008.00264.x
PII S0301-4681(09)60027-X
PMID 18452552
MeSH Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / physiology* Caenorhabditis elegans Proteins / ultrastructure Cell Polarity / genetics Cell Polarity / physiology Epithelial Cells / cytology Gene Expression Regulation, Developmental / physiology Homeostasis / physiology Intermediate Filament Proteins / biosynthesis Intermediate Filament Proteins / genetics Intermediate Filament Proteins / physiology* Intestinal Mucosa / metabolism* Intestines / cytology* Intestines / ultrastructure Microscopy, Confocal Microscopy, Electron, Transmission
IF 2.688
Times Cited 18
C.elegans tm2502