| RRC ID |
42445
|
| Author |
Ito H, Koide N, Morikawa A, Hassan F, Islam S, Tumurkhuu G, Mori I, Yoshida T, Kakumu S, Moriwaki H, Yokochi T.
|
| Title |
Augmentation of lipopolysaccharide-induced nitric oxide production by alpha-galactosylceramide in mouse peritoneal cells.
|
| Journal |
J Endotoxin Res
|
| Abstract |
The effect of alpha-galactosylceramide (alpha-GalCer) on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in mouse peritoneal cells was studied. alpha-GalCer augmented LPS-induced NO production in mouse peritoneal cells, but not in RAW 264.7 macrophage cells. alpha-GalCer augmented NO production, but not tumor necrosis factor (TNF)-alpha production in LPS-stimulated peritoneal cells. Peritoneal cells produced a significant level of interferon (IFN)-gamma in response to alpha-GalCer and anti-IFN-gamma antibody abolished the augmentation of LPS-induced NO production by alpha-GalCer. Moreover, anti-IFN-gamma antibody prevented the enhanced expression of an inducible type of NO synthase mRNA by alpha-GalCer. alpha-GalCer did not augment LPS-induced NO production in peritoneal cells from natural killer T (NKT)-deficient mice. Therefore, it was suggested that alpha-GalCer might augment LPS-induced NO production in peritoneal cells through release of IFN-gamma from NKT cells.
|
| Volume |
11(4)
|
| Pages |
213-9
|
| Published |
2005-1-1
|
| DOI |
10.1179/096805105X46628
|
| PMID |
16176657
|
| MeSH |
Animals
Cells, Cultured
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescent Antibody Technique, Direct
Fluorescent Dyes
Galactosylceramides / pharmacology*
Kinetics
Lipopolysaccharides / pharmacology*
Macrophages, Peritoneal / drug effects*
Macrophages, Peritoneal / immunology
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Mice, Knockout
Nitric Oxide / biosynthesis*
Nitrites / analysis
|
| Times Cited |
15
|
|
WOS Category
|
MEDICINE, RESEARCH & EXPERIMENTAL
IMMUNOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
