論文 - 詳細
| RRC ID | 42462 |
|---|---|
| 著者 | Chen L, Fink T, Zhang XY, Ebbesen P, Zachar V. |
| タイトル | Quantitative transcriptional profiling of ATDC5 mouse progenitor cells during chondrogenesis. |
| ジャーナル | Differentiation |
| Abstract |
During the differentiation of a mouse chondroprogenitor cell line, ATDC5, an analysis of the transcription cartilage-related genes was carried out using real-time RT-PCR in a semiquantitative fashion. A total number of 104 genes both previously linked to chondrogenesis and hitherto not associated with the development of cartilage were analyzed. Parametric statistics, and unsupervised hierarchical and K-medians clustering approaches were used to analyze the gene expression during the sequential processes of proliferation, condensation, differentiation, maturation, and hypertrophic conversion of ATDC5 cells. The obtained data provided a robust determination of expression patterns that make possible an accurate assessment of the molecular events along the chondrogenic differentiation pathway. In addition, time-course expression profiles were described for eight highly regulated genes that have not been associated with chondrogenesis as yet. These included Cryab, Rcor2, Hig1, Bnip3, Mst4, Calml4, Gng2, and Islr. |
| 巻・号 | 73(7) |
| ページ | 350-63 |
| 公開日 | 2005-9-1 |
| DOI | 10.1111/j.1432-0436.2005.00038.x |
| PII | S0301-4681(09)60346-7 |
| PMID | 16219039 |
| MeSH | Animals Cell Culture Techniques Cell Differentiation Cell Line Cell Proliferation Chondrogenesis* Clone Cells Gene Expression Profiling* Gene Expression Regulation, Developmental* Genes, Regulator Insulin / pharmacology Kinetics Mice Reverse Transcriptase Polymerase Chain Reaction Stem Cells / cytology* Transcription, Genetic* |
| IF | 2.392 |
| 引用数 | 35 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |