RRC ID 42463
著者 Wang D, Gao L.
タイトル Proteomic analysis of neural differentiation of mouse embryonic stem cells.
ジャーナル Proteomics
Abstract Mouse embryonic stem cells (mESCs) can differentiate into different types of cells, and serve as a good model system to study human embryonic stem cells (hESCs). We showed that mESCs differentiated into two types of neurons with different time courses. To determine the global protein expression changes after neural differentiation, we employed a proteomic strategy to analyze the differences between the proteomes of ES cells (E14) and neurons. Using 2-DE plus LC/MS/MS, we have generated proteome reference maps of E14 cells and derived dopaminergic neurons. Around 23 proteins with an increase or decrease in expression or phosphorylation after differentiation have been identified. We confirmed the downregulation of translationally controlled tumor protein (TCTP) and upregulation of alpha-tubulin by Western blotting. We also showed that TCTP was further downregulated in derived motor neurons than in dopaminergic neurons, and its expression level was independent of extracellular Ca(2+) concentration during neural differentiation. Potential roles of TCTP in modulating neural differentiation through binding to Ca(2+), tubulin and Na,K-ATPase, as well as the functional significance of regulation of other proteins such as actin-related protein 3 (Arp3) and Ran GTPase are discussed. This study demonstrates that proteomic tools are valuable in studying stem cell differentiation and elucidating the underlying molecular mechanisms.
巻・号 5(17)
ページ 4414-26
公開日 2005-11-1
DOI 10.1002/pmic.200401304
PMID 16222718
MeSH Animals Cell Differentiation Electrophoresis, Gel, Two-Dimensional / methods Embryo, Mammalian Enzymes / analysis Enzymes / genetics Immunoblotting Mice Nerve Tissue Proteins / analysis* Nerve Tissue Proteins / genetics Neurons / chemistry Neurons / cytology* Neurons / enzymology Phosphoproteins / analysis Phosphoproteins / genetics Phosphorylation Proteome / analysis* Proteomics / methods Stem Cells / cytology* Tumor Protein, Translationally-Controlled 1 ran GTP-Binding Protein / analysis ran GTP-Binding Protein / genetics
IF 3.254
引用数 59
WOS 分野 BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MC3T3-G2/PA6(RCB1127)