| RRC ID |
42470
|
| 著者 |
Ho Ye S, Watanabe J, Takai M, Iwasaki Y, Ishihara K.
|
| タイトル |
High functional hollow fiber membrane modified with phospholipid polymers for a liver assist bioreactor.
|
| ジャーナル |
Biomaterials
|
| Abstract |
For practical application of a liver assist system with a tissue-conjugated hollow fiber membrane (HFM) bioreactor used in an extracorporeal therapy, it would require a highly sophisticated HFM which has both hemocompatibility on one side and cytocompatibility on the other side. In this study, we present a cellulose acetate (CA) HFM modified with 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymers (PMB30 (MPC-co-n-butyl methacrylate) and PMA30 (MPC-co-methacrylic acid) for preparing a novel liver assist HFM bioreactor. A CA/PMB-PMA30 HFM modified asymmetrically on the inner and outer surface with the PMB30 and PMA30 was prepared successfully. Analysis with an X-ray photoelectron spectroscope showed that the intensity of the phosphorus atom attributed to the MPC units on the outer surface of the modified HFM was stronger than that of the inner surface. The PMA30 was immobilized on the outer surface of the CA/PMB30 blend HFM by a chemical condensation reaction. The CA/PMB-PMA30 HFM showed good water and solute permeability in comparison with the CA HFM. The morphologies of the adherent hepatocytes were round in shape in comparison with the cells that adhered on CA HFM. Furthermore, hepatocytes cultured on the inner surface of the CA/PMB-PMA30 HFM showed higher functional expression in terms of urea synthesis and albumin synthesis than that of the CA HFM.
|
| 巻・号 |
27(9)
|
| ページ |
1955-62
|
| 公開日 |
2006-3-1
|
| DOI |
10.1016/j.biomaterials.2005.09.041
|
| PII |
S0142-9612(05)00880-X
|
| PMID |
16239028
|
| MeSH |
Animals
Bioreactors*
Cells, Cultured
Hepatocytes / drug effects
Hepatocytes / metabolism
Liver, Artificial*
Membranes, Artificial*
Methacrylates / chemical synthesis
Methacrylates / chemistry*
Methacrylates / toxicity
Permeability
Phospholipids / chemistry
Phosphorylcholine / analogs & derivatives*
Phosphorylcholine / chemical synthesis
Phosphorylcholine / chemistry
Phosphorylcholine / toxicity
Polymers / chemistry
Rats
|
| IF |
10.317
|
| 引用数 |
48
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
RTH33(RCB1025) |
