| RRC ID |
42473
|
| 著者 |
Nakamura M, Gabazza EC, Imoto I, Yano Y, Taguchi O, Horiki N, Fukudome K, Suzuki K, Adachi Y.
|
| タイトル |
Anti-inflammatory effect of activated protein C in gastric epithelial cells.
|
| ジャーナル |
J Thromb Haemost
|
| Abstract |
It has been previously demonstrated that activated protein C (APC) plays an important role in the inhibition of inflammation in the gastric mucosa from patients with Helicobacter pylori infection. However, the role of gastric epithelial cells in the anti-inflammatory activity of APC remains unknown. In the present study, we evaluated the anti-inflammatory activity of APC and the expression of thrombomodulin (TM) and endothelial protein C receptor (EPCR) in gastric epithelial cells. The gastric epithelial cell lines, MKN-1 and AGS, and gastric biopsy samples from patients with and without H. pylori infection were used in the experiments. Polymerase chain reaction showed that gastric epithelial cell lines express EPCR and TM. Flow cytometry analysis also showed EPCR expression in both cells. H. pylori infection significantly increased EPCR expression compared with non-infected cells. Similar results were observed in vivo when samples from patients with and without H. pylori infection were analyzed for EPCR protein expression. Significant TM activity was found on AGS and MKN-1 cells stimulated with LPS from Escherichia coli and VacA antigen. APC was able to significantly inhibit the secretion of MCP-1 and IL-1beta induced by H. pylori homogenate in AGS cells. APC also remarkably suppressed the mRNA expression and secretion of MCP-1 from AGS cells infected with H. pylori. These results demonstrated the expression of components of the protein C pathway on gastric epithelial cells and that APC may play a critical role in the protection against gastric mucosal inflammation.
|
| 巻・号 |
3(12)
|
| ページ |
2721-9
|
| 公開日 |
2005-12-1
|
| DOI |
10.1111/j.1538-7836.2005.01635.x
|
| PII |
JTH1635
|
| PMID |
16246255
|
| MeSH |
Antigens / analysis
Antigens / genetics
Antigens / physiology
Antigens, CD
Blood Coagulation Factors / analysis
Blood Coagulation Factors / genetics
Blood Coagulation Factors / physiology
Cells, Cultured
Cytokines / metabolism
Endothelial Protein C Receptor
Endothelium, Vascular / cytology
Epithelial Cells / chemistry*
Epithelial Cells / metabolism
Glycoproteins / analysis
Glycoproteins / genetics
Glycoproteins / physiology
Helicobacter Infections / metabolism
Helicobacter Infections / pathology
Humans
Inflammation / pathology*
Protein C / metabolism
Protein C / physiology*
RNA, Messenger / analysis
Receptor, PAR-1 / physiology
Receptors, Cell Surface / analysis
Receptors, Cell Surface / genetics
Receptors, Cell Surface / physiology
Stomach / cytology*
Stomach / pathology
Thrombomodulin / analysis
Thrombomodulin / genetics
Thrombomodulin / metabolism
|
| IF |
4.157
|
| 引用数 |
10
|
|
WOS 分野
|
HEMATOLOGY
PERIPHERAL VASCULAR DISEASE
|
| リソース情報 |
| ヒト・動物細胞 |
MKN1(RCB1003) |
