論文 - 詳細
| RRC ID | 42476 |
|---|---|
| 著者 | Iwabata H, Yoshida M, Komatsu Y. |
| タイトル | Proteomic analysis of organ-specific post-translational lysine-acetylation and -methylation in mice by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies. |
| ジャーナル | Proteomics |
| Abstract |
Post-translational lysine-acetylation and -methylation are two major PTMs of lysine residues in proteins. Recently, we established pan-reactive anti-acetyllysine mouse mAbs, which can bind to Nepsilon-acetylated lysine residues in various contexts of amino acid sequences. In the present study, we established pan-reactive anti-methyllysine mouse mAbs comparable to the anti-acetyllysine ones. By using these anti-acetyllysine and -methyllysine antibodies, we found that the pattern of lysine-acetylated and -methylated proteins in mouse organs showed extreme variation from organ to organ. We selected brain and skeletal muscle as model cases to be further analyzed by 2-DE followed by Western blotting. In brain, alpha-tubulin at its basal level was found to be extremely acetylated; and alpha-enolase was shown to be a newly recognized possibly acetylated protein. NF-L protein, Hsc70, alpha-tubulin fragments, beta-actin, and brain-type creatine kinase were identified as putative lysine-methylated proteins in mouse brain. In skeletal muscle, lysine-methylation of alpha-actin and both lysine-acetylation and -methylation of muscle-type creatine kinase were found as novel putative lysine-modified proteins. The approach presented here might be useful to find novel disease markers and/or drug target molecules that would not be noticed by use of the traditional proteomic approach only. |
| 巻・号 | 5(18) |
| ページ | 4653-64 |
| 公開日 | 2005-12-1 |
| DOI | 10.1002/pmic.200500042 |
| PMID | 16247734 |
| MeSH | Acetylation Actins / metabolism Animals Antibodies, Monoclonal / biosynthesis Brain Chemistry Creatine Kinase, MM Form / analysis Cross Reactions Female Histones / metabolism Horseradish Peroxidase / immunology Hybridomas / immunology Lysine / analogs & derivatives* Lysine / immunology Lysine / metabolism* Methylation Mice Muscle Proteins / chemistry Muscle, Skeletal / chemistry Nerve Tissue Proteins / chemistry Organ Specificity Protein Processing, Post-Translational / genetics* Proteomics* Tubulin / metabolism |
| IF | 3.254 |
| 引用数 | 78 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SP2/0-Ag14(RCB0209) |