論文 - 詳細
| RRC ID | 42482 |
|---|---|
| 著者 | Mizoguchi I, Ooe Y, Hoshino S, Shimura M, Kasahara T, Kano S, Ohta T, Takaku F, Nakayama Y, Ishizaka Y. |
| タイトル | Improved gene expression in resting macrophages using an oligopeptide derived from Vpr of human immunodeficiency virus type-1. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Vpr, an accessory gene product of human immunodeficiency virus type-1, is thought to transport a viral DNA from the cytoplasm to the nucleus in resting macrophages. Previously, we reported that a peptide encompassing amino acids 52-78 of Vpr (C45D18) promotes the nuclear trafficking of recombinant proteins that are conjugated with C45D18. Here, we present evidence that C45D18, when conjugated with a six-branched cationic polymer of poly(N,N-dimethylaminopropylacrylamide)-block-oligo(4-aminostyrene) (SV: star vector), facilitates gene expression in resting macrophages. Although there was no difference between SV alone and C45D18-SV with respect to gene transduction into growing cells, C45D18-SV resulted in more than 40-fold greater expression of the exogenous gene upon transduction into chemically differentiated macrophages and human quiescent monocyte-derived macrophages. The data suggest that C45D18 contributes to improving the ability of a non-viral vector to transduce macrophages with exogenous genes and we discuss its further application. |
| 巻・号 | 338(3) |
| ページ | 1499-506 |
| 公開日 | 2005-12-23 |
| DOI | 10.1016/j.bbrc.2005.10.112 |
| PII | S0006-291X(05)02358-2 |
| PMID | 16274667 |
| MeSH | Amino Acid Sequence Cell Line, Tumor Cell Nucleus / chemistry Cell Nucleus / metabolism DNA / genetics Gene Expression / drug effects* Gene Expression / genetics Gene Products, vpr / chemistry* HIV-1 / chemistry* HIV-1 / genetics Humans Macrophages / drug effects* Macrophages / metabolism* Molecular Sequence Data Peptide Fragments / chemistry* Peptide Fragments / genetics Peptide Fragments / pharmacology* vpr Gene Products, Human Immunodeficiency Virus |
| IF | 2.985 |
| 引用数 | 16 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |