RRC ID 42482
Author Mizoguchi I, Ooe Y, Hoshino S, Shimura M, Kasahara T, Kano S, Ohta T, Takaku F, Nakayama Y, Ishizaka Y.
Title Improved gene expression in resting macrophages using an oligopeptide derived from Vpr of human immunodeficiency virus type-1.
Journal Biochem Biophys Res Commun
Abstract Vpr, an accessory gene product of human immunodeficiency virus type-1, is thought to transport a viral DNA from the cytoplasm to the nucleus in resting macrophages. Previously, we reported that a peptide encompassing amino acids 52-78 of Vpr (C45D18) promotes the nuclear trafficking of recombinant proteins that are conjugated with C45D18. Here, we present evidence that C45D18, when conjugated with a six-branched cationic polymer of poly(N,N-dimethylaminopropylacrylamide)-block-oligo(4-aminostyrene) (SV: star vector), facilitates gene expression in resting macrophages. Although there was no difference between SV alone and C45D18-SV with respect to gene transduction into growing cells, C45D18-SV resulted in more than 40-fold greater expression of the exogenous gene upon transduction into chemically differentiated macrophages and human quiescent monocyte-derived macrophages. The data suggest that C45D18 contributes to improving the ability of a non-viral vector to transduce macrophages with exogenous genes and we discuss its further application.
Volume 338(3)
Pages 1499-506
Published 2005-12-23
DOI 10.1016/j.bbrc.2005.10.112
PII S0006-291X(05)02358-2
PMID 16274667
MeSH Amino Acid Sequence Cell Line, Tumor Cell Nucleus / chemistry Cell Nucleus / metabolism DNA / genetics Gene Expression / drug effects* Gene Expression / genetics Gene Products, vpr / chemistry* HIV-1 / chemistry* HIV-1 / genetics Humans Macrophages / drug effects* Macrophages / metabolism* Molecular Sequence Data Peptide Fragments / chemistry* Peptide Fragments / genetics Peptide Fragments / pharmacology* vpr Gene Products, Human Immunodeficiency Virus
IF 2.985
Times Cited 16
Human and Animal Cells