| RRC ID |
42482
|
| 著者 |
Mizoguchi I, Ooe Y, Hoshino S, Shimura M, Kasahara T, Kano S, Ohta T, Takaku F, Nakayama Y, Ishizaka Y.
|
| タイトル |
Improved gene expression in resting macrophages using an oligopeptide derived from Vpr of human immunodeficiency virus type-1.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Vpr, an accessory gene product of human immunodeficiency virus type-1, is thought to transport a viral DNA from the cytoplasm to the nucleus in resting macrophages. Previously, we reported that a peptide encompassing amino acids 52-78 of Vpr (C45D18) promotes the nuclear trafficking of recombinant proteins that are conjugated with C45D18. Here, we present evidence that C45D18, when conjugated with a six-branched cationic polymer of poly(N,N-dimethylaminopropylacrylamide)-block-oligo(4-aminostyrene) (SV: star vector), facilitates gene expression in resting macrophages. Although there was no difference between SV alone and C45D18-SV with respect to gene transduction into growing cells, C45D18-SV resulted in more than 40-fold greater expression of the exogenous gene upon transduction into chemically differentiated macrophages and human quiescent monocyte-derived macrophages. The data suggest that C45D18 contributes to improving the ability of a non-viral vector to transduce macrophages with exogenous genes and we discuss its further application.
|
| 巻・号 |
338(3)
|
| ページ |
1499-506
|
| 公開日 |
2005-12-23
|
| DOI |
10.1016/j.bbrc.2005.10.112
|
| PII |
S0006-291X(05)02358-2
|
| PMID |
16274667
|
| MeSH |
Amino Acid Sequence
Cell Line, Tumor
Cell Nucleus / chemistry
Cell Nucleus / metabolism
DNA / genetics
Gene Expression / drug effects*
Gene Expression / genetics
Gene Products, vpr / chemistry*
HIV-1 / chemistry*
HIV-1 / genetics
Humans
Macrophages / drug effects*
Macrophages / metabolism*
Molecular Sequence Data
Peptide Fragments / chemistry*
Peptide Fragments / genetics
Peptide Fragments / pharmacology*
vpr Gene Products, Human Immunodeficiency Virus
|
| IF |
2.985
|
| 引用数 |
16
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1(RCB1189) |
