論文 - 詳細
| RRC ID | 42500 |
|---|---|
| 著者 | Kanzaki N, Terashima M, Kashimura S, Hoshino M, Ohtani S, Matsuyama S, Hoshino Y, Kogure M, Oshibe I, Endo H, Saito T, Yaginuma H, Gotoh M, Ohto H. |
| タイトル | Understanding the response of dendritic cells to activation by streptococcal preparation OK-432. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND:The streptococcal preparation OK-432 induces maturation of T cells and dendritic cells (DCs). However, the mechanisms by which OK-432 induces DC maturation are not well understood. MATERIALS AND METHODS:The effects of OK-432 and TNF-alpha on peripheral blood mononuclear cells (PBMCs) were compared. Antibody-based approaches were used to detect proteins characteristic of antigen-presenting cells and cytokines. Cytotoxicity was evaluated by measuring the release of LDH after incubation of effector and target cells. The TLR-4 levels were measured by real-time quantitative PCR. RESULTS:Changes in cell surface marker levels were detected in both treatment groups but OK-432 had a greater effect on induction of Th-1-type cytokines. Furthermore, TLR-4 mRNA was up-regulated in cells from two out of five patients in response to OK-432, but not in response to TNF-alpha. CONCLUSION:OK-432 has a more profound effect on human DCs than TNF-alpha and may act through multiple signaling pathways. |
| 巻・号 | 25(6B) |
| ページ | 4231-8 |
| 公開日 | 2005-1-1 |
| PMID | 16309221 |
| MeSH | Adult Cell Line, Tumor Cytokines / biosynthesis Cytokines / immunology Dendritic Cells / drug effects* Dendritic Cells / immunology* Humans Leukocytes, Mononuclear / drug effects Leukocytes, Mononuclear / immunology Picibanil / immunology Picibanil / pharmacology* RNA, Messenger / biosynthesis RNA, Messenger / genetics Toll-Like Receptor 4 / biosynthesis Toll-Like Receptor 4 / genetics Tumor Necrosis Factor-alpha / immunology Tumor Necrosis Factor-alpha / pharmacology Up-Regulation |
| IF | 1.994 |
| 引用数 | 1 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |