論文 - 詳細
| RRC ID | 42507 |
|---|---|
| 著者 | Ohguchi K, Akao Y, Nozawa Y. |
| タイトル | Involvement of calpain in melanogenesis of mouse B16 melanoma cells. |
| ジャーナル | Mol Cell Biochem |
| Abstract |
In the current study, the involvement of calpain, a cysteine proteinase in the regulation of melanogenesis was examined using mouse B16 melanoma cells. In response to alpha-melanocyte-stimulating hormone (a-MSH), B16 melanoma cells underwent differentiation characterized by increased melanin biosynthesis. The total calapain activity was decreased within 2 h following alpha-MSH-treatment, and restored to the initial level in 6-12 h. To further investigate the involvement of calpain in the regulation of melanogenesis, the effect of calpain inhibitors on alpha-MSH-induced melanogenesis was examined. Inhibition of calpain by either N-acetyl-Leu-Leu-norleucinal (ALLN) or calpastatin (CS) peptide blocked alpha-MSH-induced melanogenesis. The magnitude of inhibition of melanin biosynthesis was well correlated with a decrease in the activity of tyrosinase, a key regulatory enzyme in melanogenesis. Treatment of B16 cells with ALLN caused marked decrease in both tyrosinase protein and mRNA levels. These results indicate that calpain would be involved in the melanogenic signaling by modulating the expression of tyrosinase in mouse B16 melanoma cells. |
| 巻・号 | 275(1-2) |
| ページ | 103-7 |
| 公開日 | 2005-7-1 |
| DOI | 10.1007/s11010-005-1081-0 |
| PMID | 16335789 |
| MeSH | Animals Blotting, Western Calcium-Binding Proteins / pharmacology Calpain / analysis Calpain / antagonists & inhibitors Calpain / pharmacology* Cell Differentiation / drug effects Cysteine Proteinase Inhibitors / pharmacology Electrophoresis, Polyacrylamide Gel Hormones / pharmacology Indolequinones / analysis Leupeptins / pharmacology Melanins / biosynthesis Melanocytes / metabolism* Melanoma, Experimental Mice Monophenol Monooxygenase / analysis Monophenol Monooxygenase / metabolism RNA, Messenger / metabolism Tumor Cells, Cultured alpha-MSH / pharmacology |
| IF | 2.795 |
| 引用数 | 18 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma |