論文 - 詳細
| RRC ID | 42508 |
|---|---|
| 著者 | Takagi M, Suto F, Suga T, Yamada J. |
| タイトル | Sterol Regulatory Element-Binding Protein-2 modulates human brain acyl-CoA hydrolase gene transcription. |
| ジャーナル | Mol Cell Biochem |
| Abstract |
The brain shows high catalyzing activity during hydrolysis of long-chain acyl-CoAs into fatty acids and CoA-SH. Brain acyl-CoA hydrolase (BACH) is responsible for most of the long-chain acyl-CoA hydrolyzing activity in the brain and is localized exclusively in neurons. We analyzed the human BACH gene promoter, focusing on transcriptional regulation by Sterol Regulatory Element-Binding Protein-2 (SREBP-2), which is a transcription factor that activates genes involved in cholesterol biosynthesis and uptake. When the nuclear form of SREBP-2 gene was transfected into human neuroblastoma cells, transcription of a BACH gene promoter-luciferase reporter gene was activated through a sterol regulatory element (SRE) motif. Moreover, a gel shift assay demonstrated that SREBP-2 specifically bound to the SRE motif. These results suggest that transcription of the BACH gene is activated by SREBP-2. This study also provides insights into BACH function in the interaction between the metabolism of acyl-CoAs and cholesterol in neurons. |
| 巻・号 | 275(1-2) |
| ページ | 199-206 |
| 公開日 | 2005-7-1 |
| DOI | 10.1007/s11010-005-1990-y |
| PMID | 16335799 |
| MeSH | Brain / enzymology* Cell Line, Tumor Electrophoretic Mobility Shift Assay Gene Expression Regulation Genes, Reporter HeLa Cells Humans Luciferases / metabolism Neuroblastoma / pathology Palmitoyl-CoA Hydrolase / genetics* Promoter Regions, Genetic Sterol Regulatory Element Binding Protein 2 / metabolism* Transcription, Genetic* Transfection |
| IF | 2.795 |
| 引用数 | 7 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) |