論文 - 詳細
| RRC ID | 42512 |
|---|---|
| 著者 | Xia HH, He H, De Wang J, Gu Q, Lin MC, Zou B, Yu LF, Sun YW, Chan AO, Kung HF, Wong BC. |
| タイトル | Induction of apoptosis and cell cycle arrest by a specific c-Jun NH2-terminal kinase (JNK) inhibitor, SP-600125, in gastrointestinal cancers. |
| ジャーナル | Cancer Lett |
| Abstract |
The c-Jun NH(2)-terminal kinase (JNK) is activated in several tumor cell lines. The aim of this study was to determine the effects of SP-600125, a specific JNK inhibitor, on the viability, apoptosis, cell cycle distribution of gastrointestinal cancer cells, and the potential anti-tumor mechanisms. Three gastric cancer cell lines, AGS, BCG-823 and MKN-45, and three colorectal cancer cell lines, SW1116, COLO205 and HT-29, were used. Cells were treated with SP-600125, and cell viability, apoptosis and cell cycle distribution, caspase-3 activity, expression of JNK and apoptosis related proteins were detected. SP-600125 inhibited cell proliferation by 10-80% for the different cell lines, and increased apoptosis by 1.5-4.5 folds for COLO205, BCG-823, MKN-45, AGS cells. Caspase-8 and caspase-3 were involved in the induction of apoptosis. SP-600125 caused G2/M cell cycle arrest and elevation of cyclin B1 and p27(kip). The differential response in cells to SP-600125 was associated with the basal level of phosphorylated JNK2. It is concluded that SP-600125 inhibits proliferation, induces apoptosis and causes cell cycle arrest in gastrointestinal cancer cells, indicating that JNK inhibitors have an anti-tumor effect and are potential therapeutic agents for cancers. |
| 巻・号 | 241(2) |
| ページ | 268-74 |
| 公開日 | 2006-9-28 |
| DOI | 10.1016/j.canlet.2005.10.031 |
| PII | S0304-3835(05)00946-8 |
| PMID | 16337741 |
| MeSH | Anthracenes / pharmacology* Apoptosis / drug effects* Caspases / metabolism Cell Division / drug effects* Cell Proliferation / drug effects Enzyme Inhibitors / pharmacology* G2 Phase / drug effects* Gastrointestinal Neoplasms / drug therapy* Humans JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors* JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism RNA, Messenger / metabolism Tumor Cells, Cultured |
| IF | 7.36 |
| 引用数 | 31 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001 |