| RRC ID |
42520
|
| 著者 |
Yamamoto H, Tani Y, Kobayashi K, Taketani Y, Sato T, Arai H, Morita K, Miyamoto K, Pike JW, Kato S, Takeda E.
|
| タイトル |
Alternative promoters and renal cell-specific regulation of the mouse type IIa sodium-dependent phosphate cotransporter gene.
|
| ジャーナル |
Biochim Biophys Acta
|
| Abstract |
The type IIa sodium-dependent phosphate cotransporter (NPT2a) expressed in renal proximal tubules represents an important determinant in maintaining inorganic phosphate (Pi) homeostasis. In the present study, we identified two variant transcripts of the mouse NPT2a gene, Npt2a-v1 and Npt2a-v2, characterized by the presence of alternative first exons (either exon 1A or exon 1B). The chromosomal structure analysis revealed that the Npt2a gene comprises of two promoters (promoters 1 and 2) and 14 exons, and spans approximately 17 kb. Quantitative PCR analysis showed that renal mRNA levels of both the variants markedly decreased in X-linked vitamin D-resistant hypophosphatemic rickets (Hyp) mice compared to normal littermates. Interestingly, transcriptional activity of a reporter gene, containing Npt2a promoters 1 and 2, was renal cell-specifically increased by 1alpha, 25(OH)2D3 and its analogs. The deletion analysis revealed that the CAAT box in the Npt2a promoter 2 is important for the 1alpha, 25(OH)2D3-dependent renal cell-specific activation of the reporter gene. These data suggested that two alternative promoters control the renal expression of Npt2a gene and both Npt2a variant transcripts are down regulated in Hyp mice.
|
| 巻・号 |
1732(1-3)
|
| ページ |
43-52
|
| 公開日 |
2005-12-30
|
| DOI |
10.1016/j.bbaexp.2005.11.003
|
| PII |
S0167-4781(05)00274-5
|
| PMID |
16380173
|
| MeSH |
5' Flanking Region / genetics
5' Untranslated Regions / genetics
Animals
Base Sequence
COS Cells
Caco-2 Cells
Calcitriol / pharmacology
Cells, Cultured
Chlorocebus aethiops
Chromosomes, Mammalian / metabolism
Exons / genetics
Gene Expression Regulation* / drug effects
Humans
Kidney / cytology*
Kidney / metabolism*
Mice
Mice, Inbred C57BL
Mice, Obese
Molecular Sequence Data
Opossums
Organ Specificity
Promoter Regions, Genetic / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sequence Deletion / genetics
Sodium-Phosphate Cotransporter Proteins, Type IIa / genetics*
|
| IF |
3.411
|
| 引用数 |
17
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539)
CACO-2(RCB0988) |
