| RRC ID |
42521
|
| 著者 |
Verbrugge A, Rijkers ES, de Ruiter T, Meyaard L.
|
| タイトル |
Leukocyte-associated Ig-like receptor-1 has SH2 domain-containing phosphatase-independent function and recruits C-terminal Src kinase.
|
| ジャーナル |
Eur J Immunol
|
| Abstract |
Most inhibitory receptors in the immune system contain one or several immunoreceptor tyrosine-based inhibitory motifs (ITIM) and recruit the SH2 domain-containing phosphatases SHP-1, SHP-2 and/or SHIP, which are generally believed to be essential for the inhibitory function. However, it has not been systematically investigated whether ITIM-bearing receptors exert their function through alternative interactions. Here we describe that leukocyte-associated Ig-like receptor (LAIR)-1 has inhibitory function in DT40 chicken B cells that lack both SHP-1 and SHP-2. In addition, we found that LAIR-1 did not recruit SHIP upon phosphorylation. Thus, LAIR-1 can function independently from SH2 domain-containing phosphatases and must recruit at least one other signaling molecule. Using a yeast-tri-hybrid system, we found that phosphorylated LAIR-1 bound the C-terminal Src kinase (Csk). The interaction required the SH2 domain of Csk and phosphorylation of the tyrosine in the N-terminal ITIM of LAIR-1. We propose that Csk is an additional player in the regulation of the immune system by ITIM-bearing receptors.
|
| 巻・号 |
36(1)
|
| ページ |
190-8
|
| 公開日 |
2006-1-1
|
| DOI |
10.1002/eji.200535226
|
| PMID |
16380958
|
| MeSH |
Animals
B-Lymphocytes / immunology*
B-Lymphocytes / metabolism
Blotting, Western
CSK Tyrosine-Protein Kinase
Cell Line
Chickens
Humans
Mice
Phosphoric Monoester Hydrolases
Protein-Tyrosine Kinases / immunology
Protein-Tyrosine Kinases / metabolism*
Receptors, Antigen, B-Cell / immunology
Receptors, Antigen, B-Cell / metabolism
Receptors, Immunologic / immunology
Receptors, Immunologic / metabolism*
Two-Hybrid System Techniques
src Homology Domains / immunology*
src-Family Kinases
|
| IF |
4.404
|
| 引用数 |
44
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
SHP1^(-) DT40(RCB1466)
SHP2^(-) DT40(RCB1502)
SHP1^(-)/SHP2^(-) DT40(RCB1501)
SHIP^(-) DT40(RCB1645) |
