論文 - 詳細
| RRC ID | 42522 |
|---|---|
| 著者 | Takata MK, Yamaguchi F, Nakanose K, Watanabe Y, Hatano N, Tsukamoto I, Nagata M, Izumori K, Tokuda M. |
| タイトル | Neuroprotective effect of D-psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 muM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 muM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione. |
| 巻・号 | 100(5) |
| ページ | 511-6 |
| 公開日 | 2005-11-1 |
| DOI | 10.1263/jbb.100.511 |
| PII | S1389-1723(05)70502-8 |
| PMID | 16384789 |
| MeSH | Adrenergic Agents / pharmacology* Animals Apoptosis / drug effects* Fructose / pharmacology* Glutathione / biosynthesis Humans Neuroprotective Agents / pharmacology* Oxidopamine / pharmacology* PC12 Cells Parkinson Disease / drug therapy Parkinson Disease / metabolism Rats Up-Regulation / drug effects |
| IF | 2.366 |
| 引用数 | 46 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |