RRC ID 42548
著者 Tao RH, Kawate H, Ohnaka K, Ishizuka M, Hagiwara H, Takayanagi R.
タイトル Opposite effects of alternative TZF spliced variants on androgen receptor.
ジャーナル Biochem Biophys Res Commun
Abstract We previously demonstrated that testicular zinc-finger protein (TZF) was a corepressor of the androgen receptor (AR). In the present study, we further showed that TZF-L, an alternative spliced variant of TZF, enhanced transactivation function of AR. Deletion analysis of TZF-L revealed that its N-terminus, which almost corresponded to that of TZF, but not its C-terminus was able to interact with AR. Additional analysis suggested that TZF and TZF-L were able to form both homodimers and heterodimers. TZF-L inhibited the homodimer formation of TZF and the intranuclear dot formation of TZF. We propose that in the unique regulation system of AR-mediated transactivation, two spliced isoforms of TZF act as coactivator and corepressor, respectively.
巻・号 341(2)
ページ 515-21
公開日 2006-3-10
DOI 10.1016/j.bbrc.2005.12.213
PII S0006-291X(06)00045-3
PMID 16446156
MeSH Alternative Splicing* Amino Acid Motifs Animals COS Cells Cell Line, Tumor Chlorocebus aethiops Dimerization Gene Deletion Genes, Reporter Humans Immunoblotting Immunoprecipitation Mice Microscopy, Confocal Models, Biological NIH 3T3 Cells Plasmids / metabolism Protein Isoforms Protein Structure, Tertiary Receptors, Androgen / metabolism* Repressor Proteins / chemistry Repressor Proteins / metabolism* Transcriptional Activation Transfection
IF 2.985
引用数 10
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報
ヒト・動物細胞 COS-7(RCB0539)