論文 - 詳細
| RRC ID | 42557 |
|---|---|
| 著者 | Tao RH, Kawate H, Wu Y, Ohnaka K, Ishizuka M, Inoue A, Hagiwara H, Takayanagi R. |
| タイトル | Testicular zinc finger protein recruits histone deacetylase 2 and suppresses the transactivation function and intranuclear foci formation of agonist-bound androgen receptor competitively with TIF2. |
| ジャーナル | Mol Cell Endocrinol |
| Abstract |
We previously reported that testicular zinc finger protein (TZF) is a corepressor for androgen receptor (AR). The present study demonstrated that a central portion (amino acids 512-663) of TZF, TZF(512-663), is responsible for both binding to AR and repressing the transactivation. TZF recruited endogenous histone deacetylase 2 (HDAC2) and formed a complex with agonist-bound AR. Imaging analyses showed that TZF and TZF(512-663) were recruited by AR and simultaneously impaired distinct AR foci formation. Quantification of the foci number using a three-dimensional imaging method revealed that the number of intranuclear AR foci was related to its transactivation activity. Moreover, increased levels of TZF dissociated a coactivator, TIF2, from the AR foci and vice versa. These results indicate that the ligand-dependent transactivation function of AR is quantitatively related to its intranuclear foci formation, and suggest that corepressors, such as TZF, act on these intranuclear events competitively with coactivators. |
| 巻・号 | 247(1-2) |
| ページ | 150-65 |
| 公開日 | 2006-3-9 |
| DOI | 10.1016/j.mce.2005.12.052 |
| PII | S0303-7207(06)00003-7 |
| PMID | 16469430 |
| MeSH | 5-alpha-Dihydroprogesterone / pharmacology Androgens Animals Cell Line Chlorocebus aethiops Cloning, Molecular Histone Deacetylase 2 Histone Deacetylases / metabolism* Humans Male Mice Mutation Nuclear Receptor Coactivator 2 / metabolism* Protein Binding Receptors, Androgen / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Transcriptional Activation* |
| IF | 3.871 |
| 引用数 | 15 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |