RRC ID 42557
著者 Tao RH, Kawate H, Wu Y, Ohnaka K, Ishizuka M, Inoue A, Hagiwara H, Takayanagi R.
タイトル Testicular zinc finger protein recruits histone deacetylase 2 and suppresses the transactivation function and intranuclear foci formation of agonist-bound androgen receptor competitively with TIF2.
ジャーナル Mol Cell Endocrinol
Abstract We previously reported that testicular zinc finger protein (TZF) is a corepressor for androgen receptor (AR). The present study demonstrated that a central portion (amino acids 512-663) of TZF, TZF(512-663), is responsible for both binding to AR and repressing the transactivation. TZF recruited endogenous histone deacetylase 2 (HDAC2) and formed a complex with agonist-bound AR. Imaging analyses showed that TZF and TZF(512-663) were recruited by AR and simultaneously impaired distinct AR foci formation. Quantification of the foci number using a three-dimensional imaging method revealed that the number of intranuclear AR foci was related to its transactivation activity. Moreover, increased levels of TZF dissociated a coactivator, TIF2, from the AR foci and vice versa. These results indicate that the ligand-dependent transactivation function of AR is quantitatively related to its intranuclear foci formation, and suggest that corepressors, such as TZF, act on these intranuclear events competitively with coactivators.
巻・号 247(1-2)
ページ 150-65
公開日 2006-3-9
DOI 10.1016/j.mce.2005.12.052
PII S0303-7207(06)00003-7
PMID 16469430
MeSH 5-alpha-Dihydroprogesterone / pharmacology Androgens Animals Cell Line Chlorocebus aethiops Cloning, Molecular Histone Deacetylase 2 Histone Deacetylases / metabolism* Humans Male Mice Mutation Nuclear Receptor Coactivator 2 / metabolism* Protein Binding Receptors, Androgen / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism* Transcriptional Activation*
IF 3.871
引用数 15
WOS 分野 ENDOCRINOLOGY & METABOLISM CELL BIOLOGY
リソース情報
ヒト・動物細胞 COS-7(RCB0539)