RRC ID 42559
Author Yoshida T, Oide N, Sakamoto T, Yotsumoto S, Negishi Y, Tsuchiya S, Aramaki Y.
Title Induction of cancer cell-specific apoptosis by folate-labeled cationic liposomes.
Journal J Control Release
Abstract We have previously reported that cationic liposomes themselves can induce apoptosis in macrophages and lymphocytes. In this paper, we attempted the cancer cell-specific delivery of cationic liposomes and the induction of apoptosis utilizing this characteristic. Cationic liposomes composed of stearylamine (SA-liposomes) induced apoptosis in human nasopharyngeal epidermoid carcinoma cells (KB cells) overexpressing the folate receptor and human fibroblasts (WI-38 cells) with no folate receptor, without showing selectivity. To recruit liposomes to cancer cells and induce apoptosis, we focused on the folate receptor and prepared folic acid-labeled liposomes using polyethyleneglycol (PEG) (folate-PEG-liposomes). Folate-PEG-liposomes showed selectivity and induced apoptosis in KB cells, but not WI-38 cells. The apoptosis occurred in a dose-dependent manner. Furthermore, folate-PEG-liposomes appear to associate with KB cells via the folate receptor, whereas SA-liposomes may associate with cells through electrostatic interactions. To confirm the contribution of the folate receptor to apoptosis of KB cells induced by folate-PEG-liposomes, the effect of folic acid on the apoptosis was examined. The addition of free folic acid drastically suppressed the apoptosis of KB cells and the percentage of cells with hypodiploid nuclei returned to the control level. Taken together, cationic liposomes labeled with folate bound to KB cells via folate receptors and, interestingly, the cationic liposomes themselves could cause apoptosis in cancer cells.
Volume 111(3)
Pages 325-32
Published 2006-4-10
DOI 10.1016/j.jconrel.2005.12.016
PII S0168-3659(06)00004-6
PMID 16478640
MeSH Amines / chemistry Amines / metabolism Apoptosis* Carrier Proteins / biosynthesis Carrier Proteins / genetics Carrier Proteins / metabolism Cations Cell Line, Tumor* Folate Receptors, GPI-Anchored Folic Acid* / chemistry Humans Liposomes* / chemistry Nasopharyngeal Neoplasms Polyethylene Glycols / chemistry Receptors, Cell Surface / biosynthesis Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism
IF 7.727
Times Cited 19
Human and Animal Cells WI-38