| RRC ID |
42559
|
| 著者 |
Yoshida T, Oide N, Sakamoto T, Yotsumoto S, Negishi Y, Tsuchiya S, Aramaki Y.
|
| タイトル |
Induction of cancer cell-specific apoptosis by folate-labeled cationic liposomes.
|
| ジャーナル |
J Control Release
|
| Abstract |
We have previously reported that cationic liposomes themselves can induce apoptosis in macrophages and lymphocytes. In this paper, we attempted the cancer cell-specific delivery of cationic liposomes and the induction of apoptosis utilizing this characteristic. Cationic liposomes composed of stearylamine (SA-liposomes) induced apoptosis in human nasopharyngeal epidermoid carcinoma cells (KB cells) overexpressing the folate receptor and human fibroblasts (WI-38 cells) with no folate receptor, without showing selectivity. To recruit liposomes to cancer cells and induce apoptosis, we focused on the folate receptor and prepared folic acid-labeled liposomes using polyethyleneglycol (PEG) (folate-PEG-liposomes). Folate-PEG-liposomes showed selectivity and induced apoptosis in KB cells, but not WI-38 cells. The apoptosis occurred in a dose-dependent manner. Furthermore, folate-PEG-liposomes appear to associate with KB cells via the folate receptor, whereas SA-liposomes may associate with cells through electrostatic interactions. To confirm the contribution of the folate receptor to apoptosis of KB cells induced by folate-PEG-liposomes, the effect of folic acid on the apoptosis was examined. The addition of free folic acid drastically suppressed the apoptosis of KB cells and the percentage of cells with hypodiploid nuclei returned to the control level. Taken together, cationic liposomes labeled with folate bound to KB cells via folate receptors and, interestingly, the cationic liposomes themselves could cause apoptosis in cancer cells.
|
| 巻・号 |
111(3)
|
| ページ |
325-32
|
| 公開日 |
2006-4-10
|
| DOI |
10.1016/j.jconrel.2005.12.016
|
| PII |
S0168-3659(06)00004-6
|
| PMID |
16478640
|
| MeSH |
Amines / chemistry
Amines / metabolism
Apoptosis*
Carrier Proteins / biosynthesis
Carrier Proteins / genetics
Carrier Proteins / metabolism
Cations
Cell Line, Tumor*
Folate Receptors, GPI-Anchored
Folic Acid* / chemistry
Humans
Liposomes* / chemistry
Nasopharyngeal Neoplasms
Polyethylene Glycols / chemistry
Receptors, Cell Surface / biosynthesis
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism
|
| IF |
7.727
|
| 引用数 |
19
|
|
WOS 分野
|
CHEMISTRY, MULTIDISCIPLINARY
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
WI-38 |
