| RRC ID |
42563
|
| Author |
Elloumi I, Kobayashi R, Funabashi H, Mie M, Kobatake E.
|
| Title |
Construction of epidermal growth factor fusion protein with cell adhesive activity.
|
| Journal |
Biomaterials
|
| Abstract |
A novel protein for controlling cellular functions was constructed by combining functional units of various proteins. The Arg-Gly-Asp (RGD) sequence functioning as a cell adhesive function, an epidermal growth factor (EGF) as a cell growth function, and a hydrophobic sequence (E12) as an efficient assembling function, were combined and incorporated into one molecule. The fusion protein, designated ERE-EGF, was produced in Escherichia coli and purified with affinity chromatography using a His-tag. The ERE-EGF coated on an unmodified hydrophobic surface of a cell-culture plate (through the hydrophobic E12 moiety) retained both cell adhesive activity (through the RGD sequence) and cell growth activity (through the EGF moiety).
|
| Volume |
27(18)
|
| Pages |
3451-8
|
| Published |
2006-6-1
|
| DOI |
10.1016/j.biomaterials.2006.02.003
|
| PII |
S0142-9612(06)00121-9
|
| PMID |
16497372
|
| MeSH |
Amino Acid Sequence
Cell Adhesion / drug effects*
Escherichia coli / genetics
Hydrophobic and Hydrophilic Interactions
Molecular Sequence Data
Oligopeptides / chemistry
Oligopeptides / pharmacology
Recombinant Fusion Proteins / chemistry*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / pharmacology*
Tumor Cells, Cultured
|
| IF |
10.317
|
| Times Cited |
45
|
|
WOS Category
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| Resource |
| Human and Animal Cells |
A549(RCB0098) |
