RRC ID |
42567
|
著者 |
Noguchi A, Watanabe N, Fujimaki R, Kitamura T, Hayashizaki Y, Miyaki S, Tezuka K, Hozumi N.
|
タイトル |
Use of the SST-REX method for the identification of genes expressed at the condensation stage of chondrogenic cell line ATDC5.
|
ジャーナル |
J Bone Miner Metab
|
Abstract |
In endochondral ossification, chondrogenesis precedes bone development. Cartilage differentiation is initiated by the formation of chondrogenic cell condensates. Thus, it is essential to investigate genes expressed at the condensation stage for a better understanding of chondrogenesis and ossification. To this end, we constructed a cDNA library from the mRNA fraction derived from a chondrogenic cell line (ATDC5) at the cell condensation stage by using the signal sequence trap by retrovirus-mediated expression (SST-REX) method. We obtained 486 factor (IL-3)-independent clones by screening 5.7 x 10(3) clones. DNA sequencing analysis of the clones identified genes encoding 157 known proteins and 4 novel proteins. These 4 genes encoding novel proteins were expressed not only in chondrogenic ATDC5 cells but also in osteogenic MC3T3-E1 and myogenic C2C12 cells. The mRNA expression level of 1 of the 4 clones increased at the calcification stage of the differentiation of MC3T3-E1 cells. The results demonstrate that the SST-REX method is a useful experimental system to identify genes involved in the complicated mechanisms of bone formation.
|
巻・号 |
24(2)
|
ページ |
153-7
|
公開日 |
2006-1-1
|
DOI |
10.1007/s00774-005-0662-z
|
PMID |
16502123
|
MeSH |
Animals
Blotting, Northern
Bone and Bones / metabolism
Cartilage / metabolism*
Cell Differentiation
Cell Line
Chondrocytes / metabolism
Chondrogenesis
Cloning, Molecular
DNA, Complementary / metabolism
Gene Expression*
Gene Library
Interleukin-3 / metabolism
Mice
RNA / metabolism
RNA, Messenger / metabolism
Retroviridae / genetics
|
IF |
2.297
|
引用数 |
0
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
MEDICINE, RESEARCH & EXPERIMENTAL
|
リソース情報 |
ヒト・動物細胞 |
ATDC5(RCB0565) |