RRC ID 426
Author Göbel V, Barrett PL, Hall DH, Fleming JT.
Title Lumen morphogenesis in C. elegans requires the membrane-cytoskeleton linker erm-1.
Journal Dev. Cell
Abstract Epithelial tubes are basic building blocks of complex organs, but their architectural requirements are not well understood. Here we show that erm-1 is a unique C. elegans ortholog of the ERM family of cytoskeleton-membrane linkers, with an essential role in lumen morphogenesis. ERM-1 localizes to the luminal membranes of those tubular organ epithelia which lack stabilization by cuticle. RNA interference (RNAi), a germline deletion, and overexpression of erm-1 cause cystic luminal phenotypes in these epithelia. Confocal and ultrastructural analyses indicate that erm-1 functions directly in apical membrane morphogenesis, rather than in epithelial polarity and junction assembly as has been previously proposed for ERMs. We also show that act-5/cytoplasmic actin and sma-1/beta-H-spectrin are required for lumen formation and functionally interact with erm-1. Our findings suggest that there are common structural constraints on the architecture of diverse organ lumina.
Volume 6(6)
Pages 865-73
Published 2004-6
DOI 10.1016/j.devcel.2004.05.018
PII S1534-5807(04)00171-6
PMID 15177034
MeSH Animals Body Patterning / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / isolation & purification Caenorhabditis elegans Proteins / metabolism* Cell Membrane / metabolism* Cell Membrane / ultrastructure Cell Polarity / genetics Cytoskeletal Proteins / genetics Cytoskeletal Proteins / isolation & purification Cytoskeletal Proteins / metabolism* Cytoskeleton / metabolism* Cytoskeleton / ultrastructure Drosophila Proteins / genetics Drosophila Proteins / metabolism Epithelial Cells / metabolism Epithelial Cells / ultrastructure Microscopy, Electron Organogenesis / genetics RNA Interference Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Spectrin / genetics Spectrin / metabolism
IF 9.616
Times Cited 92
WOS Category DEVELOPMENTAL BIOLOGY CELL BIOLOGY
Resource
C.elegans tm677