RRC ID 42602
著者 Morita M, Banno Y, Dohjima T, Nozawa S, Fushimi K, Fan DG, Ohno T, Miyazawa K, Liu N, Shimizu K.
タイトル Mu-calpain is involved in the regulation of TNF-alpha-induced matrix metalloproteinase-3 release in a rheumatoid synovial cell line.
ジャーナル Biochem Biophys Res Commun
Abstract Calpain is secreted by intra-articular synovial cells and degrades the main components of cartilage matrix proteins, proteoglycan, and collagen, causing cartilage destruction. Matrix metalloproteinase-3 (MMP-3) has also been detected in synovial fluid and serum, and is involved in the development and progression of rheumatoid arthritis by degradation of the extracellular matrix and cartilage destruction. To investigate the relationship between calpain and MMP-3 in rheumatic inflammation, we utilized the rheumatic synovial cell line, MH7A. Tumor necrosis factor (TNF-alpha) stimulation-induced increased expression of mu-calpain, m-calpain, and MMP-3 in these cells, as well as the release of calpain and MMP-3 into the culture medium. The calpain inhibitors, ALLN (calpain inhibitor I) and calpeptin, did not affect the intracellular expression of MMP-3, but reduced the secretion of MMP-3 in a concentration-dependent manner. Down-regulation of mu- but not m-calpain by small interfering RNAs abolished TNF-alpha-induced MMP-3 release from the synovial cells. These findings suggest that calpain, particularly mu-calpain, regulates MMP-3 release by rheumatic synovial cells, in addition to exerting its own degradative action on cartilage.
巻・号 343(3)
ページ 937-42
公開日 2006-5-12
DOI 10.1016/j.bbrc.2006.02.155
PII S0006-291X(06)00466-9
PMID 16574073
MeSH Arthritis, Rheumatoid / enzymology* Calpain / antagonists & inhibitors Calpain / biosynthesis Calpain / physiology* Cell Line Cysteine Proteinase Inhibitors / pharmacology Humans Matrix Metalloproteinase 3 / biosynthesis* RNA Interference Synovial Membrane / cytology Synovial Membrane / enzymology* Tumor Necrosis Factor-alpha / pharmacology* Up-Regulation
IF 2.985
引用数 8
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 MH7A(RCB1512)