RRC ID 42604
Author Nagayoshi K, Ohkawa H, Yorozu K, Higuchi M, Higashi S, Kubota N, Fukui H, Imai N, Gojo S, Hata J, Kobayashi Y, Umezawa A.
Title Increased mobilization of c-kit+ Sca-1+ Lin- (KSL) cells and colony-forming units in spleen (CFU-S) following de novo formation of a stem cell niche depends on dynamic, but not stable, membranous ossification.
Journal J Cell Physiol
Abstract Stem cells are thought to inhabit in a unique microenvironment, known as "niche," in which they undergo asymmetric cell divisions that results in reproducing both stem cells and progenies to maintain various tissues throughout life. The cells of osteoblastic lineage have been identified as a key participant in regulating the number of hematopoietic stem cells (HSCs). HSCs receive their regulatory messages from the microenvironment in the bone marrow. This would account for a reason why the localization of hematopoiesis is usually restricted in the bone marrow. To clarify the above possibility we employed a cell implantation-based strategy with a unique osteoblast cell line (KUSA-A1) derived from a C3H/He mouse. The implantation of KUSA-A 1 cells resulted in the generation of ectopic bones in the subcutaneous tissues of the athymic BALB/c nu/nu mice. Subsequently the mice obtained a greater amount of the bone marrow than normal mice, and they showed an increased number of HSCs. These results indicate that the newly generated osteoblasts-derived ectopic bones are responsible for the increase in the number of the HSC population. Furthermore, the increased number of HSCs directly correlates with both the magnitude of dynamic osteogenic process and the size of the newly generated bone or "niche."
Volume 208(1)
Pages 188-94
Published 2006-7-1
DOI 10.1002/jcp.20652
PMID 16575918
MeSH Animals Antigens, Ly / analysis Bone Marrow Cells / cytology Bone Marrow Cells / physiology Bone and Bones Cell Count Cell Line Cell Movement / physiology* Cells, Cultured Choristoma / immunology Choristoma / pathology Choristoma / physiopathology Cytokines / metabolism Femur / cytology Femur / immunology Femur / physiology Flow Cytometry Gene Expression Regulation Hematopoiesis / physiology Hematopoietic Stem Cells / chemistry Hematopoietic Stem Cells / cytology* Hematopoietic Stem Cells / immunology Hematopoietic Stem Cells / physiology* Major Histocompatibility Complex / genetics Major Histocompatibility Complex / physiology Membrane Proteins / analysis Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Osteoblasts / cytology Osteoblasts / immunology Osteoblasts / physiology Osteoblasts / transplantation Osteogenesis / physiology* Proto-Oncogene Proteins c-kit / analysis Spleen / chemistry Spleen / cytology* Spleen / immunology Spleen / physiology* Stem Cells / physiology* Subcutaneous Tissue / immunology Subcutaneous Tissue / pathology Subcutaneous Tissue / physiopathology Transplants
IF 5.546
Times Cited 7
Human and Animal Cells ST2(RCB0224)