RRC ID 42606
Author Chen L, Fink T, Ebbesen P, Zachar V.
Title Temporal transcriptome of mouse ATDC5 chondroprogenitors differentiating under hypoxic conditions.
Journal Exp Cell Res
Abstract The formation of cartilage takes place in vivo in an environment of reduced oxygen tension. To study the effect of hypoxia on the process of chondrogenesis, ATDC5 mouse chondroprogenitor cells were induced to differentiate by the addition of insulin and cultured under ambient and hypoxic conditions corresponding to 21% and 1% O2 in the gas phase, respectively. The production of extracellular proteoglycans as well as the transcriptional profile of 104 selected genes was determined by real-time RT-PCR. Hypoxia alone induced early chondrogenesis as evidenced by the synthesis of glycosaminoglycans and expression of aggrecan and collagen type II genes. Surprisingly, however, hypoxic incubation of insulin-treated cells delayed and suppressed the insulin-mediated early chondrogenesis and almost completely blocked hypertrophic differentiation. Analysis of the gene expression yielded several clues as to the mechanisms involved. In addition, a group of genes was identified that have not previously been associated with hypoxia, including Ak4, Akt3, Col X, Fmod, Ier3, IGFbp4, MafF, Mxi1, Rcor2, Rras, Sox6, Tnni2, Wnt5a, and Zfp313.
Volume 312(10)
Pages 1727-44
Published 2006-6-10
DOI 10.1016/j.yexcr.2006.02.013
PII S0014-4827(06)00047-4
PMID 16580664
MeSH Aggrecans Animals Cartilage* / cytology Cartilage* / embryology Cell Differentiation / physiology Cell Line Chondrogenesis / physiology* Chondroitin Sulfate Proteoglycans / genetics Chondroitin Sulfate Proteoglycans / metabolism Collagen Type II / genetics Collagen Type II / metabolism Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Gene Expression* Gene Expression Profiling Glycosaminoglycans / metabolism Hypoxia* Insulin / metabolism Lectins, C-Type / genetics Lectins, C-Type / metabolism Mice Molecular Sequence Data Oligonucleotide Array Sequence Analysis Oxygen / metabolism Stem Cells / cytology Stem Cells / physiology* Transcription, Genetic*
IF 3.383
Times Cited 20
Human and Animal Cells ATDC5(RCB0565)