Reference - Detail
| RRC ID | 42607 |
|---|---|
| Author | Ogawa M, Yanoma S, Nagashima Y, Okamoto N, Ishikawa H, Haruki A, Miyagi E, Takahashi T, Hirahara F, Miyagi Y. |
| Title | Paradoxical discrepancy between the serum level and the placental intensity of PP5/TFPI-2 in preeclampsia and/or intrauterine growth restriction: possible interaction and correlation with glypican-3 hold the key. |
| Journal | Placenta |
| Abstract |
There have been controversies whether maternal serum placental protein 5 (PP5)/tissue factor pathway inhibitor (TFPI)-2 is increased in the patients with preeclampsia and/or intrauterine growth restriction (IUGR). Here, we have estimated the serum PP5/TFPI-2 in these patients by a sandwich enzyme-linked immunosorbent assay with a newly developed monoclonal antibody, coupled with placental immunohistochemical studies of their placentae with semiquantitative scoring. Serum PP5/TFPI-2 level was significantly elevated only in the patients with preeclampsia alone (p=0.033), while PP5/TFPI-2 was detected significantly less intensely in the placentae of the same patients (p=0.035) in immunohistochemistry, as compared to Controls. A proteoglycan present on the placental villous surface, glypican-3, showed the same pattern of staining as PP5/TFPI-2, and there was a positive correlation (C.I.=0.506, p=0.004) between the immunohistochemical scores for these. Further experiments using HepG2 cells transfected with PP5/TFPI-2 suggested that glypican-3 could anchor PP5/TFPI-2 on the placental villi. A possibility that a decrease in glypican-3 in the placenta increases the outflow of PP5/TFPI-2, which in turn increases its serum level, was proposed. Preeclampsia and IUGR, often regarded to have the same pathological basis in common, showed distinct distributions of PP5/TFPI-2, which could be a clue to elucidate the pathogenesis of preeclampsia and IUGR. |
| Volume | 28(2-3) |
| Pages | 224-32 |
| Published | 2007-1-1 |
| DOI | 10.1016/j.placenta.2006.01.023 |
| PII | S0143-4004(06)00028-2 |
| PMID | 16580726 |
| MeSH | Adult Female Fetal Growth Retardation / blood Fetal Growth Retardation / metabolism* Glycoproteins / blood Glycoproteins / metabolism* Glypicans / metabolism* Humans Immunohistochemistry Infant, Newborn Male Placenta / metabolism* Pre-Eclampsia / blood Pre-Eclampsia / metabolism* Pregnancy Syndecan-1 / metabolism |
| IF | 3.177 |
| Times Cited | 28 |
| WOS Category | OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |