| RRC ID |
42630
|
| 著者 |
Muzandu K, Ishizuka M, Sakamoto KQ, Shaban Z, El Bohi K, Kazusaka A, Fujita S.
|
| タイトル |
Effect of lycopene and beta-carotene on peroxynitrite-mediated cellular modifications.
|
| ジャーナル |
Toxicol Appl Pharmacol
|
| Abstract |
Peroxynitrite formed by the reaction of superoxide and nitric oxide is a highly reactive species with a role in various pathological processes such as cancer, chronic inflammation, and cardiovascular and neurological diseases. In the present study, the effect of the carotenoids, lycopene and beta-carotene, on peroxynitrite-mediated modifications in plasmid DNA as well as cellular DNA and proteins were investigated. In pUC18 plasmid DNA, these carotenoids strongly inhibited DNA strand breaks caused by peroxynitrite generated from 3-morpholinosydnonimine (SIN-1). SIN-1 was also used to determine effects on DNA damage and protein tyrosine nitration in Chinese hamster lung fibroblasts. SIN-1 dose-dependently increased nitration of proteins in cells above basal levels as determined by Western blotting. This nitration was inhibited in the presence of the uric acid as well as lycopene. Physiological concentrations (0.31-10 microM) of lycopene and beta-carotene also had protective effects on DNA damage, as measured by the comet assay. Lycopene significantly reduced DNA damage particularly, in the median range of concentrations (2.5 microM). The protective effects of lycopene and beta-carotene could be due to their scavenging of reactive oxygen (ROS) and/or nitrogen species (RNS) as they reduce the amount of intracellular ROS/RNS produced following treatment with SIN-1 by as much as 47.5% and 42.4%, respectively. The results obtained in this study suggest that carotenoids may alleviate some of the deleterious effects of peroxynitrite and possibly other reactive nitrogen species as well in vivo.
|
| 巻・号 |
215(3)
|
| ページ |
330-40
|
| 公開日 |
2006-9-15
|
| DOI |
10.1016/j.taap.2006.03.006
|
| PII |
S0041-008X(06)00104-9
|
| PMID |
16647730
|
| MeSH |
Animals
Antioxidants / pharmacology*
Carotenoids / pharmacology*
Cell Line
Cell Survival
Comet Assay
Cricetinae
Cricetulus
DNA Damage / drug effects*
Lycopene
Molsidomine / analogs & derivatives
Molsidomine / toxicity
Nitric Oxide Donors / toxicity
Peroxynitrous Acid / toxicity*
Tyrosine / analogs & derivatives
Tyrosine / metabolism
beta Carotene / pharmacology*
|
| IF |
3.347
|
| 引用数 |
29
|
|
WOS 分野
|
TOXICOLOGY
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
V79(RCB0008) |
