RRC ID 42639
Author Shen H, Sun WH, Xue QP, Wu J, Cheng YL, Ding GX, Fu HY, Tsuji S, Kawano S.
Title Influences of Helicobacter pylori on cyclooxygenase-2 expression and prostaglandinE2 synthesis in rat gastric epithelial cells in vitro.
Journal J Gastroenterol Hepatol
Abstract BACKGROUND AND AIM:It is known that cyclooxygenase (COX)-2 is over expressed in gastrointestinal neoplasia and Helicobacter pylori (H. pylori) infection is causally linked to gastric cancer. The present study aimed to elucidate the effects of H. pylori on COX-2 expression and prostaglandinE(2) (PGE(2)) production in a gastric epithelial cell line derived from normal rat gastric mucosa (RGM1).
METHOD:H. pylori water extracts were prepared from a supernatant of the H. pylori suspension in distilled water. RGM1 cells were cultured with H. pylori water extracts at the final concentration of 2.5, 5, 10 microg/mL for 24 h. For the time sequence study, RGM1 cells were cultured with 10 microg/mL H. pylori water extracts for 0, 6, 12, 24 and 48 h. COX-1 and COX-2 expression in the RGM1 cells was analyzed by western blotting. The levels of PGE(2) in the cultured media were measured by enzyme immunoassay.
RESULTS:H. pylori did not affect COX-1 expression; whereas COX-2 expression increased by six-fold at 24 h after incubation of RGM1 cells with 10 microg/mL H. pylori water extracts. The increase in COX-2 expression was evident after 12 h of incubation; reached a peak at 24 h and declined at 48 h. H. pylori dose dependently increased COX-2 expression and PGE(2) synthesis in RGM1 cells.
CONCLUSION:H. pylori induces COX-2 expression and increases PGE(2) synthesis in RGM1 cells in vitro. These results indicate that H. pylori-associated gastric carcinogenesis may depend on COX-2 expression.
Volume 21(4)
Pages 754-8
Published 2006-4-1
DOI 10.1111/j.1440-1746.2006.04299.x
PMID 16677165
MeSH Animals Cells, Cultured Cyclooxygenase 2 / metabolism* Dinoprostone / metabolism* Epithelial Cells / metabolism* Epithelial Cells / microbiology* Gastric Mucosa / metabolism* Gastric Mucosa / microbiology* Gene Expression / physiology Helicobacter pylori / physiology* Rats Rats, Wistar
IF 3.437
Times Cited 16
Human and Animal Cells RGM1(RCB0876)