| RRC ID |
42642
|
| 著者 |
Huang JY, Chen WH, Chang YL, Wang HT, Chuang WT, Lee SC.
|
| タイトル |
Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation.
|
| ジャーナル |
Nucleic Acids Res
|
| Abstract |
Chromatin-modifying factors play key roles in transcription, DNA replication and DNA repair. Post-translational modification of these proteins is largely responsible for regulating their activity. The FACT (facilitates chromatin transcription) complex, a heterodimer of hSpt16 and SSRP1, is a chromatin structure modulator whose involvement in transcription and DNA replication has been reported. Here we show that nucleosome binding activity of FACT complex is regulated by poly(ADP-ribosyl)ation. hSpt16, the large subunit of FACT, is poly(ADP-ribosyl)ated by poly(ADP-ribose) polymerase-1 (PARP-1) resulting from physical interaction between these two proteins. The level of hSpt16 poly(ADP-ribosyl)ation is elevated after genotoxic treatment and coincides with the activation of PARP-1. The enhanced hSpt16 poly(ADP-ribosyl)ation level correlates with the dissociation of FACT from chromatin in response to DNA damage. Our findings suggest that poly(ADP-ribosyl)ation of hSpt16 by PARP-1 play regulatory roles for FACT-mediated chromatin remodeling.
|
| 巻・号 |
34(8)
|
| ページ |
2398-407
|
| 公開日 |
2006-1-1
|
| DOI |
10.1093/nar/gkl241
|
| PII |
34/8/2398
|
| PMID |
16682447
|
| PMC |
PMC1458519
|
| MeSH |
Cell Cycle Proteins / metabolism*
Chromatin / metabolism
Chromatin Assembly and Disassembly
DNA Damage
DNA-Binding Proteins / metabolism
HeLa Cells
High Mobility Group Proteins / metabolism
Humans
Nucleosomes / metabolism*
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases / metabolism*
Transcription Factors / metabolism*
Transcriptional Elongation Factors / metabolism
|
| IF |
11.502
|
| 引用数 |
46
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
