論文 - 詳細
| RRC ID | 42642 |
|---|---|
| 著者 | Huang JY, Chen WH, Chang YL, Wang HT, Chuang WT, Lee SC. |
| タイトル | Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Chromatin-modifying factors play key roles in transcription, DNA replication and DNA repair. Post-translational modification of these proteins is largely responsible for regulating their activity. The FACT (facilitates chromatin transcription) complex, a heterodimer of hSpt16 and SSRP1, is a chromatin structure modulator whose involvement in transcription and DNA replication has been reported. Here we show that nucleosome binding activity of FACT complex is regulated by poly(ADP-ribosyl)ation. hSpt16, the large subunit of FACT, is poly(ADP-ribosyl)ated by poly(ADP-ribose) polymerase-1 (PARP-1) resulting from physical interaction between these two proteins. The level of hSpt16 poly(ADP-ribosyl)ation is elevated after genotoxic treatment and coincides with the activation of PARP-1. The enhanced hSpt16 poly(ADP-ribosyl)ation level correlates with the dissociation of FACT from chromatin in response to DNA damage. Our findings suggest that poly(ADP-ribosyl)ation of hSpt16 by PARP-1 play regulatory roles for FACT-mediated chromatin remodeling. |
| 巻・号 | 34(8) |
| ページ | 2398-407 |
| 公開日 | 2006-1-1 |
| DOI | 10.1093/nar/gkl241 |
| PII | 34/8/2398 |
| PMID | 16682447 |
| PMC | PMC1458519 |
| MeSH | Cell Cycle Proteins / metabolism* Chromatin / metabolism Chromatin Assembly and Disassembly DNA Damage DNA-Binding Proteins / metabolism HeLa Cells High Mobility Group Proteins / metabolism Humans Nucleosomes / metabolism* Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases / metabolism* Transcription Factors / metabolism* Transcriptional Elongation Factors / metabolism |
| IF | 11.502 |
| 引用数 | 46 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 10H-2(RCB1142) |