| RRC ID |
42669
|
| 著者 |
Watari A, Takaki K, Higashiyama S, Li Y, Satomi Y, Takao T, Tanemura A, Yamaguchi Y, Katayama I, Shimakage M, Miyashiro I, Takami K, Kodama K, Yutsudo M.
|
| タイトル |
Suppression of tumorigenicity, but not anchorage independence, of human cancer cells by new candidate tumor suppressor gene CapG.
|
| ジャーナル |
Oncogene
|
| Abstract |
Previously, we isolated a series of cell lines from a human diploid fibroblast lineage as a model for multistep tumorigenesis in humans. After passaging a single LT-transfected fibroblast clone, differently progressed cell lines were obtained, including immortalized, anchorage-independent and tumorigenic cell lines. In the present paper, we analysed the gene expression profiles of these model cell lines, and observed that expression of the CapG protein was lost in the tumorigenic cell line. To examine the possibility that loss of CapG protein expression was required for tumorigenic progression, we transfected CapG cDNA into the tumorigenic cell line and tested for tumor-forming ability in nude mice. Results showed that ectopic expression of CapG suppressed tumorigenicity, but not growth in soft agar or liquid medium. We also found that certain cancer cell lines including stomach cancer, lung cancer and melanoma had also lost CapG expression. One such cancer cell line AZ521 also became non-tumorigenic after the introduction of CapG cDNA. Moreover, we showed that CapG expression was repressed in small-cell lung cancer tissues. Together, our findings indicated that CapG is a new tumor suppressor gene involved in the tumorigenic progression of certain cancers.
|
| 巻・号 |
25(56)
|
| ページ |
7373-80
|
| 公開日 |
2006-11-30
|
| DOI |
10.1038/sj.onc.1209732
|
| PII |
1209732
|
| PMID |
16767159
|
| MeSH |
Animals
Blotting, Southern
Blotting, Western
Cell Adhesion / physiology
Cell Line, Tumor
Cell Movement / physiology
Cell Transformation, Neoplastic*
Culture Media
Genes, Tumor Suppressor*
Humans
Mice
Mice, Nude
Microfilament Proteins / genetics
Microfilament Proteins / physiology*
Neoplasms / pathology*
Nuclear Proteins / genetics
Nuclear Proteins / physiology*
|
| IF |
7.971
|
| 引用数 |
30
|
|
WOS 分野
|
GENETICS & HEREDITY
ONCOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
