RRC ID 4269
Author Muzzini DM, Plevani P, Boulton SJ, Cassata G, Marini F.
Title Caenorhabditis elegans POLQ-1 and HEL-308 function in two distinct DNA interstrand cross-link repair pathways.
Journal DNA Repair (Amst.)
Abstract DNA interstrand cross-links (ICLs) are highly cytotoxic DNA lesions hindering DNA replication and transcription. Whereas in bacteria and yeast the molecular mechanisms involved in ICL repair are genetically well dissected, the scenario in multicellular organisms remains unclear. Here, we report that the two new mus308 genes, polq-1 and hel-308 are involved in ICL repair in Caenorhabditis elegans. After treatment with ICL agents, a decrease in survival and an increase in checkpoint-induced cell-cycle arrest and apoptosis of germ cells is observed in mutants of both genes. Although sensitive to ICL agents and to a minor extent to IR, cytological and epistatic analyses suggest that polq-1 and hel-308 are involved in different DNA repair pathways. While hel-308 functions in a Fanconi anemia-dependent pathway, polq-1 has a role in a novel distinct and brc-1 (CeBRCA1)-dependent ICL repair process in metazoans.
Volume 7(6)
Pages 941-50
Published 2008-6-1
DOI 10.1016/j.dnarep.2008.03.021
PII S1568-7864(08)00126-2
PMID 18472307
MeSH Animals Base Sequence Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics DNA Primers DNA Repair* DNA-Directed DNA Polymerase / metabolism* Polymerase Chain Reaction RNA Interference
IF 4.461
Times Cited 48
WOS Category TOXICOLOGY GENETICS & HEREDITY
Resource
C.elegans tm2026 tm2171 tm2134 tm1145 tm1298 tm2134