| RRC ID |
42698
|
| Author |
Zhang J, Iwai S, Tsugehara T, Takeda M.
|
| Title |
MbIDGF, a novel member of the imaginal disc growth factor family in Mamestra brassicae, stimulates cell proliferation in two lepidopteran cell lines without insulin.
|
| Journal |
Insect Biochem Mol Biol
|
| Abstract |
Imaginal disc growth factor (IDGF) is a soluble polypeptide growth factor that was first identified from the conditioned medium of Drosophilia imaginal disc C1.8+ cells. Working with insulin, IDGF stimulated the growth of cultured imaginal disk cells, which suggested that IDGF might function as a cofactor of Drosophila insulin or insulin like peptide. Here we report a new member of the IDGF family, named MbIDGF, from the cabbage armyworm, Mamestra brassicae. Using a cloned cDNA of MbIDGF, recombinant MbIDGF protein was expressed in baculovirus-infected Sf9 cells and purified. Without insulin, the recombinant MbIDGF protein stimulated cell growth of SES-MaBr-4 and NIAS-MaBr-93 cell lines that were derived from the fat bodies and hemocytes of M. brassicae, in a dose-dependent manner. The saturation of growth stimulation by MbIDGF was attained for the two types of cells at 80 ng/ml (0.8 nM) and 300 ng/ml (6 nM), respectively. The results suggest that MbIDGF may stimulate the growth of lepidopteran cells by a new mechanism without associating with the insulin pathway.
|
| Volume |
36(7)
|
| Pages |
536-46
|
| Published |
2006-7-1
|
| DOI |
10.1016/j.ibmb.2006.04.002
|
| PII |
S0965-1748(06)00090-7
|
| PMID |
16835019
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Blotting, Western
Cell Line
Cell Proliferation*
Cloning, Molecular
DNA Primers
DNA, Complementary
Dose-Response Relationship, Drug
Growth Substances / chemistry
Growth Substances / genetics
Growth Substances / physiology*
Insulin / pharmacology*
Lepidoptera / cytology*
Molecular Sequence Data
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
|
| IF |
3.827
|
| Times Cited |
19
|
|
WOS Category
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
