RRC ID 42701
Author Yamaguchi T, Saneyoshi M, Takahashi H, Hirokawa S, Amano R, Liu X, Inomata M, Maruyama T.
Title Synthetic nucleosides and nucleotides. 43. Inhibition of vertebrate telomerases by carbocyclic oxetanocin g (C.OXT-G) triphosphate analogues and influence of C.OXT-G treatment on telomere length in human HL60 cells.
Journal Nucleosides Nucleotides Nucleic Acids
Abstract Telomerase, responsible for telomere synthesis, is expressed in approximately 90% of human tumor cells but seldom in normal somatic cells. In this study, inhibition by carbocyclic oxetanocin G triphosphate (C. OXT-GTP) and its analogues was investigated in order to clarify the susceptibility of telomerase to various nucleotide analogues. C. OXT-GTP competitively inhibited telomerase activity with respect to dGTP However, C. OXT-GTP had a potent inhibitory effect on DNA polymerase alpha. It was examined whether the nucleoside (C. OXT-G) was able to alter telomere length in cultured human HL60 cells. Contrary to expectation, long-term treatment with 10 microM C. OXT-G was found to cause telomere lengthening.
Volume 25(4-6)
Pages 539-51
Published 2006
DOI 10.1080/15257770600684217
PMID 16838844
MeSH Animals Guanine / analogs & derivatives* Guanine / chemistry Guanine / pharmacology Guanosine Triphosphate / chemistry* HL-60 Cells Humans Molecular Structure Salmon / metabolism Telomerase / antagonists & inhibitors* Telomere / drug effects* Telomere / genetics Telomere / metabolism
IF 1.167
Times Cited 17
Human and Animal Cells