RRC ID 42716
Author Lee KH, Choi EM.
Title Rubus coreanus Miq. extract promotes osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells.
Journal Am. J. Chin. Med.
Abstract To prevent bone loss that occurs with increasing age, certain nutritional and pharmacological factors are needed. In the present study, the ethanol extract from the fruit of Rubus coreanus Miq. (RCE) was investigated for its effect on the function of osteoblastic MC3T3-E1 cells. RCE (10approximately50 microg/ml) caused a significant elevation in cell viability, alkaline phosphatase (ALP) activity, collagen content, and osteocalcin secretion in the cells. The effect of RCE (50 microg/ml) in increasing cell viability, ALP activity, and collagen content was prevented by the presence of 10(-6) M cycloheximide and 10(-6) M tamoxifen, suggesting that RCE's effect results from a newly synthesized protein component and might be partly involved in estrogen action. We then examined the effect of RCE on the H(2)O(2)-induced apoptosis and production of local factors in osteoblasts. Treatment with RCE (10approximately50 microg/ml) decreased the 0.2 mM H(2)O(2)-induced apoptosis and production of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and nitric oxide (NO) in osteoblasts. Our data indicate that the enhancement of osteoblast function by Rubus coreanus Miq. may result in the prevention of osteoporosis and inflammatory bone diseases.
Volume 34(4)
Pages 643-54
Published 2006
DOI 10.1142/S0192415X0600417X
PII S0192415X0600417X
PMID 16883635
MeSH 3T3 Cells Alkaline Phosphatase / metabolism Animals Apoptosis / drug effects Cell Differentiation / drug effects* Cell Survival / drug effects Collagen / biosynthesis Cycloheximide / pharmacology Cytokines / biosynthesis Dose-Response Relationship, Drug Fruit / chemistry Hydrogen Peroxide / toxicity Mice Nitric Oxide / biosynthesis Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Osteocalcin / metabolism Plant Extracts / pharmacology* Rosaceae / chemistry* Tamoxifen / pharmacology
IF 3.51
Times Cited 18
Human and Animal Cells