RRC ID 42723
著者 Maniratanachote R, Minami K, Katoh M, Nakajima M, Yokoi T.
タイトル Dephosphorylation of ribosomal protein P0 in response to troglitazone-induced cytotoxicity.
ジャーナル Toxicol Lett
Abstract Troglitazone (TRO)-induced cytotoxicity was investigated in HepG2 cells. The cells were exposed to TRO as well as rosiglitazone (RSG) at concentrations of 0, 25, 50 and 75 microM for 48 h. Total proteins were separated by two-dimensional electrophoresis and visualized by silver staining. We focused on a protein spot at an approximate molecular weight of 35 kDa and isoelectric point (pI) of 5.7, which appeared only with the cytotoxic concentrations (50 and 75 microM) of TRO, but not with the low concentration (25 microM) of TRO or any concentrations of RSG. This protein spot was subjected to amino acid sequence analysis and identified as ribosomal protein P0 (P0). Interestingly, without any significant induction of its protein and mRNA, P0 was dephosphorylated depending on the concentration- and time-dependent manner of TRO-induced cytotoxicity. Pretreatment with a general caspase inhibitor, Z-VAD.fmk, prevented cleavage of caspase-3 but demonstrated a slight improvement of cytotoxicity induced by TRO. Thus, these effects could not prevent the dephosphorylation of P0. Our results strongly suggest that a post-translational modification, dephosphorylation, of P0 is associated with TRO-induced cytotoxicity.
巻・号 166(3)
ページ 189-99
公開日 2006-10-25
DOI 10.1016/j.toxlet.2006.07.303
PII S0378-4274(06)01156-8
PMID 16893617
MeSH Amino Acid Sequence Antineoplastic Agents / toxicity* Blotting, Western Caspases / metabolism Cell Line, Tumor Cell Survival / drug effects Chromans / toxicity* Echocardiography Humans Hydrogen Peroxide / toxicity Immunoprecipitation Isoelectric Focusing Molecular Sequence Data Molecular Weight Oxidants / toxicity Phosphoproteins / chemistry Phosphoproteins / metabolism* Phosphorylation Proteomics Reverse Transcriptase Polymerase Chain Reaction Ribosomal Proteins / chemistry Ribosomal Proteins / metabolism* Signal Transduction / physiology Thiazolidinediones / toxicity* Troglitazone
IF 3.569
引用数 6
WOS 分野 TOXICOLOGY
リソース情報
ヒト・動物細胞 Hep G2