Reference - Detail
| RRC ID | 42735 |
|---|---|
| Author | Ohgusu T, Hamase K, Tanaka H, Shoyama Y, Zaitsu K. |
| Title | High-throughput determination of free D-aspartic acid in mammals by enzyme immunoassay using specific monoclonal antibody. |
| Journal | Anal Biochem |
| Abstract |
A method for rapid determination of free D-aspartic acid (D-Asp) in mammals has been established using a highly specific mouse monoclonal antibody against D-Asp for the first time. An anti-D-Asp monoclonal antibody was obtained by the immunization of bovine-serum-albumin-conjugated D-Asp to BALB/c mice. The obtained antibody has a high specificity toward D-Asp but shows a slight cross-reactivity to all other D- and L- amino acids including L-Asp. The calibration range of the competitive enzyme linked immunosorbent assay (ELISA) is 0.016-16 micromol/mL D-Asp in rat serum samples. The precisions of this method were evaluated by inter-plate and intraplate assays, and the relative standard deviation values were 4.8% and 4.5%, respectively. The values of D-Asp determined by the present ELISA have a good correlation to those determined by high-performance liquid chromatography with the correlation coefficient of 0.963. Using this ELISA, the time course of D-Asp in the rat serum after intravenous administration was successfully demonstrated. The present method provides a simple and high-throughput determination of D-Asp in mammals, and is a useful tool for clarifying the physiological roles and diagnostic values of this D-amino acid. |
| Volume | 357(1) |
| Pages | 15-20 |
| Published | 2006-10-1 |
| DOI | 10.1016/j.ab.2006.06.039 |
| PII | S0003-2697(06)00490-8 |
| PMID | 16920063 |
| MeSH | Amino Acid Sequence Animals Antibodies, Monoclonal / immunology* Aspartic Acid / blood* Aspartic Acid / chemistry Calibration Cattle Enzyme-Linked Immunosorbent Assay / methods* Female Injections, Intravenous Mice Mice, Inbred BALB C Molecular Sequence Data Ovalbumin / chemistry Rats Sensitivity and Specificity Serum Albumin, Bovine / chemistry Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Factors |
| IF | 2.877 |
| Times Cited | 4 |
| WOS Category | CHEMISTRY, ANALYTICAL BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | SP2/0-Ag14(RCB0209) |