RRC ID 42747
Author Hirasawa N, Torigoe M, Kano K, Ohuchi K.
Title Involvement of Sp1 in lipopolysaccharide-induced expression of HDC mRNA in RAW 264 cells.
Journal Biochem Biophys Res Commun
Abstract The involvement of Sp1 in the lipopolysaccharide (LPS)-induced transcription of HDC mRNA in the mouse macrophage-like cell line RAW 264 was analyzed. LPS increased the levels of HDC mRNA 4 h after the stimulation in a concentration-dependent manner. Mithramycin A, an inhibitor of the binding of the Sp family to the GC box, reduced the LPS-induced increase in the levels of HDC mRNA at 4 h and HDC protein at 8 h in a concentration-dependent manner. By conducting electrophoretic mobility shift assays, we found that one of the transcription factors binding to the DNA probe containing the GC box sequence of the mouse HDC gene promoter region was Sp1, and that levels of Sp1-DNA probe complexes were increased by stimulation with LPS although the protein levels of Sp1 were not changed. These results suggested that Sp1 is one of the transcription factors that regulate the LPS-induced expression of HDC in RAW 264 cells.
Volume 349(2)
Pages 833-7
Published 2006-10-20
DOI 10.1016/j.bbrc.2006.08.104
PII S0006-291X(06)01930-9
PMID 16949047
MeSH Animals Cell Line Cell Nucleus / metabolism Dose-Response Relationship, Drug Histidine Decarboxylase / chemistry* Histidine Decarboxylase / metabolism Lipopolysaccharides / metabolism* Lipopolysaccharides / pharmacology Macrophages / metabolism Mast Cells / metabolism Mice Plicamycin / analogs & derivatives Plicamycin / pharmacology Promoter Regions, Genetic RNA, Messenger / metabolism Sp1 Transcription Factor / chemistry Sp1 Transcription Factor / physiology* Time Factors
IF 2.985
Times Cited 12
Human and Animal Cells RAW 264(RCB0535)