| RRC ID |
42767
|
| 著者 |
Kinoshita K, Iwasaki H, Uzui H, Ueda T.
|
| タイトル |
Candin family antifungal agent micafungin (FK463) modulates the inflammatory cytokine production stimulated by lipopolysaccharide in THP-1 cells.
|
| ジャーナル |
Transl Res
|
| Abstract |
Systemic inflammatory response syndrome (SIRS) and sepsis have been considered forms of hypercytokinemia in critically ill patients and immunocompromized hosts. It has been reported that some antimicrobial agents, including antifungal agents, not only have an antibiotic effect, but also they affect the host's immunological response. Immunofunctional cells, including monocytes and macrophages, were examined to determine whether they are influenced by the newly synthesized candin family antifungal agent micafungin (MCFG) using the human monocytic cell line THP-1 stimulated with lipopolysaccharide (LPS) as a model of hypercytokinetic conditions. LPS-induced production of TNF-alpha (tumor necrosis factor-alpha) and interleukin-8 (IL-8) in THP-1 cells was significantly suppressed dose-dependently by MCFG, although high concentrations of MCFG may reach toxic levels. It was clarified that MCFG inhibits the LPS-induced expression of TNF-alpha in THP-1 cells at the mRNA (messenger ribonucleic acid) level. In conclusion, administration of MCFG had an immunomodulatory effect on the host by reducing levels of TNF-alpha and IL-8. The effectiveness of MCFG in modulating hypercytokinemia is due not only to its direct antifungal effect, but also to the modulation of cytokine production in macrophages that regulates immunological activity and inflammation.
|
| 巻・号 |
148(4)
|
| ページ |
207-13
|
| 公開日 |
2006-10-1
|
| DOI |
10.1016/j.trsl.2006.07.001
|
| PII |
S1931-5244(06)00300-8
|
| PMID |
17002923
|
| MeSH |
Antifungal Agents / pharmacology*
Cell Line, Tumor
Dose-Response Relationship, Drug
Drug Combinations
Echinocandins
Fluconazole / pharmacology
Gene Expression / drug effects
Humans
Interleukin-8 / genetics
Interleukin-8 / metabolism*
Lipopeptides
Lipopolysaccharides / pharmacology*
Lipoproteins / pharmacology*
Macrophages / drug effects
Macrophages / metabolism
Micafungin
Monocytes / drug effects*
Monocytes / metabolism
Peptides, Cyclic / pharmacology*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / metabolism*
|
| IF |
5.411
|
| 引用数 |
11
|
|
WOS 分野
|
MEDICAL LABORATORY TECHNOLOGY
MEDICINE, RESEARCH & EXPERIMENTAL
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1(RCB1189) |
