RRC ID 42769
Author Susa T, Sato T, Ono T, Kato T, Kato Y.
Title Cofactor CLIM2 promotes the repressive action of LIM homeodomain transcription factor Lhx2 in the expression of porcine pituitary glycoprotein hormone alpha subunit gene.
Journal Biochim Biophys Acta
Abstract We have cloned a porcine orthologue of cofactor CLIM2 (Ldb1/NLI) from the porcine pituitary cDNA library by protein-protein interaction with the Yeast Two-Hybrid System using porcine Lhx2 as a bait protein. Porcine CLIM2 shows a high identity (99%) in the dimerization domain, nuclear localization signal and LIM binding domain with those of man and mouse. The expression of CLIM2 gene in the anterior pituitary lobe was detected during the porcine fetal and postnatal period by RT-PCR analysis, suggesting that this protein is constitutively expressing and plays a basic role in the anterior pituitary. Transfection assay to the pituitary tumor derived LbetaT2 cells, and the Chinese hamster ovary cells demonstrated that CLIM2 acts as a corepressor of the porcine Lhx2 function. Interestingly, CLIM2 alone apparently repressed the high level of alphaGSU gene expression in LbetaT2 cells. These data suggest that CLIM2 is a basic factor in the pituitary development and function, and plays the role of repressor to modify the function of Lhx2 on the alphaGSU gene expression.
Volume 1759(8-9)
Pages 403-9
Published 2006-1-1
DOI 10.1016/j.bbaexp.2006.08.004
PII S0167-4781(06)00135-7
PMID 17005264
MeSH Amino Acid Sequence Animals Base Sequence CHO Cells Cricetinae DNA, Complementary / genetics DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism* Female Gene Expression Regulation, Developmental Glycoprotein Hormones, alpha Subunit / genetics* Homeodomain Proteins / metabolism* Humans Male Mice Molecular Sequence Data Pituitary Gland, Anterior / embryology Pituitary Gland, Anterior / growth & development Pituitary Gland, Anterior / metabolism Pregnancy Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Swine Transcription Factors / metabolism* Transfection
IF 3.411
Times Cited 11
Human and Animal Cells CHO