論文 - 詳細
| RRC ID | 42787 |
|---|---|
| 著者 | Patanasethanont D, Nagai J, Yumoto R, Murakami T, Sutthanut K, Sripanidkulchai BO, Yenjai C, Takano M. |
| タイトル | Effects of Kaempferia parviflora extracts and their flavone constituents on P-glycoprotein function. |
| ジャーナル | J Pharm Sci |
| Abstract |
The purpose of this study was to examine the effects of extracts and flavone derivatives from the rhizome of Kaempferia parviflora on P-glycoprotein (P-gp)-mediated transport in LLC-GA5-COL150, a transfectant cell line of a porcine kidney epithelial cell line LLC-PK1 with human MDR1 cDNA. Ethanol extract obtained from Kaempferia parviflora rhizome significantly increased the accumulation of rhodamine 123 and daunorubicin, P-gp substrates, in LLC-GA5-COL150 cells, but not in LLC-PK1 cells. The aqueous extract also increased the accumulation in LLC-GA5-COL150 cells with lower potency than the ethanol extract. The effects of flavone derivatives isolated from the rhizome of Kaempferia parviflora on P-gp function were examined. Among six flavones tested, 3,5,7,3',4'-pentamethoxyflavone most potently increased the accumulation of rhodamine 123 and daunorubicin in LLC-GA5-COL150 cells in a concentration-dependent manner. In addition, 5,7-dimethoxyflavone to lesser degree increased rhodamine 123 accumulation in LLC-GA5-COL150 cells. In contrast, the other four flavone derivatives had no significant effect on the accumulation of rhodamine 123 in LLC-GA5-COL150 cells in a concentration range tested. These results indicate that extracts and flavone derivatives from the rhizome of Kaempferia parviflora can inhibit P-gp function, which may be useful for overcoming P-gp-mediated multidrug resistance and improving the oral bioavailability of anticancer agents. |
| 巻・号 | 96(1) |
| ページ | 223-33 |
| 公開日 | 2007-1-1 |
| DOI | 10.1002/jps.20769 |
| PII | S0022-3549(16)32155-4 |
| PMID | 17031860 |
| MeSH | ATP Binding Cassette Transporter, Subfamily B, Member 1 / drug effects* ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism Animals Antibiotics, Antineoplastic / metabolism Daunorubicin / metabolism Dose-Response Relationship, Drug Drug Resistance, Neoplasm / drug effects* Ethanol / chemistry Flavones / isolation & purification Flavones / pharmacology* Flavonoids / pharmacology Fluorescent Dyes / metabolism Humans LLC-PK1 Cells Plant Extracts / chemistry Plant Extracts / pharmacology Rhizome Rhodamine 123 / metabolism Solvents / chemistry Swine Time Factors Transfection Water / chemistry Zingiberaceae* |
| IF | 2.997 |
| 引用数 | 55 |
| WOS 分野 | CHEMISTRY, MEDICINAL PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LLC-GA5-CoL150(RCB0871) |