| RRC ID |
42807
|
| 著者 |
Chung SW, Chung HY, Toriba A, Kameda T, Tang N, Kizu R, Hayakawa K.
|
| タイトル |
An environmental quinoid polycyclic aromatic hydrocarbon, acenaphthenequinone, modulates cyclooxygenase-2 expression through reactive oxygen species generation and nuclear factor kappa B activation in A549 cells.
|
| ジャーナル |
Toxicol Sci
|
| Abstract |
Diesel exhaust particles (DEPs) contain oxygen-containing polycyclic aromatic hydrocarbons (PAHs) called quinoid PAHs. Some quinoid PAHs generate free radicals as they undergo enzymatic and nonenzymatic redox cycling with their corresponding semiquinone radicals. Reactive oxygen species (ROS) produced by these reactions can cause severe oxidative stress connected with inflammatory processing. Although humans and animals are continuously exposed to these chemicals in the environment, little is known about which quinoid PAHs are active. In this study, we estimated the intracellular ROS production and nuclear factor kappa B (NF-kappaB) translocation in A549 cells exposed to isomers of quinoid PAHs having two to four rings. We found that both acenaphthenequinone (AcQ) and 9,10-phenanthrenequinone (PQ) enhanced ROS generation and that AcQ translocated NF-kappaB from the cytosol to the nucleus. However, PQ, which has been reported to induce apoptosis, did not influence NF-kappaB activation. In addition, AcQ induced cyclooxygenase-2 (COX-2) expression which is a key enzyme in the inflammatory processing involved in the activation of NF-kappaB. Upregulation of NF-kappaB and COX-2 expression by AcQ treatment was suppressed by the antioxidant N-acetylcysteine (NAC). These results provide that AcQ might play an important role in human lung inflammatory diseases as an air pollutant.
|
| 巻・号 |
95(2)
|
| ページ |
348-55
|
| 公開日 |
2007-2-1
|
| DOI |
10.1093/toxsci/kfl150
|
| PII |
kfl150
|
| PMID |
17082565
|
| MeSH |
Acenaphthenes / chemistry
Acenaphthenes / toxicity*
Cell Line, Tumor
Cyclooxygenase 2 / biosynthesis*
Dose-Response Relationship, Drug
Environmental Pollutants / chemistry
Environmental Pollutants / toxicity*
Humans
Isomerism
Molecular Structure
NF-kappa B / metabolism*
Oxidative Stress / drug effects*
Reactive Oxygen Species / metabolism*
|
| IF |
3.703
|
| 引用数 |
44
|
|
WOS 分野
|
TOXICOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
