RRC ID 4284
Author Rasmussen JP, English K, Tenlen JR, Priess JR.
Title Notch signaling and morphogenesis of single-cell tubes in the C. elegans digestive tract.
Journal Dev. Cell
Abstract During organogenesis of the C. elegans digestive system, epithelial cells within a cyst-like primordium develop diverse shapes through largely unknown mechanisms. We here analyze two adjacent, dorsal epithelial cells, called pm8 and vpi1, that remodel their shapes and apical junctions to become donut-shaped, or toroidal, single-cell tubes. pm8 and vpi1 delaminate from the dorsal cyst epithelium and migrate ventrally, across the midline of the cyst, on a transient tract of laminin. pm8 appears to encircle the midline by wrapping around finger-like projections from neighboring cells. Finally, pm8 and vpi1 self-fuse to become toroids by expressing AFF-1 and EFF-1, two fusogens that are each sufficient to promote crossfusion between other cell types. Notch signaling in pm8 induces AFF-1 expression, while simultaneously repressing EFF-1 expression; vpi1 expresses EFF-1 independent of Notch. Thus, the adjacent pm8 and vpi1 cells express different fusogens, allowing them to self-fuse into separate, single-cell tubes while avoiding crossfusion.
Volume 14(4)
Pages 559-69
Published 2008-4
DOI 10.1016/j.devcel.2008.01.019
PII S1534-5807(08)00047-6
PMID 18410731
PMC PMC2435507
MeSH Animals Body Patterning* Caenorhabditis elegans* / anatomy & histology Caenorhabditis elegans* / embryology Caenorhabditis elegans* / metabolism Cell Fusion Cell Movement Cell Polarity Cell Shape Epithelial Cells / cytology Epithelial Cells / metabolism Gastrointestinal Tract* / cytology Gastrointestinal Tract* / embryology Gastrointestinal Tract* / metabolism Humans Laminin / metabolism Morphogenesis / physiology* Receptors, Notch / genetics Receptors, Notch / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Signal Transduction / physiology Transgenes
IF 9.616
Times Cited 36
C.elegans tm2214