論文 - 詳細
| RRC ID | 42844 |
|---|---|
| 著者 | Kamei Y, Suganami T, Kohda T, Ishino F, Yasuda K, Miura S, Ezaki O, Ogawa Y. |
| タイトル | Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner. |
| ジャーナル | FEBS Lett |
| Abstract |
Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues. |
| 巻・号 | 581(1) |
| ページ | 91-6 |
| 公開日 | 2007-1-9 |
| DOI | 10.1016/j.febslet.2006.12.002 |
| PII | S0014-5793(06)01432-3 |
| PMID | 17182038 |
| MeSH | Adipocytes / metabolism* Adipocytes / pathology Adipose Tissue / metabolism* Adipose Tissue / pathology Alleles* Alternative Splicing Animals Cell Line Cell Size Exons Gene Expression Regulation* Genomic Imprinting* Humans Mice Mice, Obese Neoplasms / genetics Neoplasms / metabolism Neoplasms / pathology Obesity / genetics Obesity / metabolism* Obesity / pathology Protein Isoforms / biosynthesis Protein Isoforms / genetics Proteins / genetics Proteins / metabolism* Transgenes |
| IF | 3.057 |
| 引用数 | 36 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |