| RRC ID |
42844
|
| Author |
Kamei Y, Suganami T, Kohda T, Ishino F, Yasuda K, Miura S, Ezaki O, Ogawa Y.
|
| Title |
Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner.
|
| Journal |
FEBS Lett
|
| Abstract |
Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues.
|
| Volume |
581(1)
|
| Pages |
91-6
|
| Published |
2007-1-9
|
| DOI |
10.1016/j.febslet.2006.12.002
|
| PII |
S0014-5793(06)01432-3
|
| PMID |
17182038
|
| MeSH |
Adipocytes / metabolism*
Adipocytes / pathology
Adipose Tissue / metabolism*
Adipose Tissue / pathology
Alleles*
Alternative Splicing
Animals
Cell Line
Cell Size
Exons
Gene Expression Regulation*
Genomic Imprinting*
Humans
Mice
Mice, Obese
Neoplasms / genetics
Neoplasms / metabolism
Neoplasms / pathology
Obesity / genetics
Obesity / metabolism*
Obesity / pathology
Protein Isoforms / biosynthesis
Protein Isoforms / genetics
Proteins / genetics
Proteins / metabolism*
Transgenes
|
| IF |
3.057
|
| Times Cited |
36
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
