RRC ID 42844
Author Kamei Y, Suganami T, Kohda T, Ishino F, Yasuda K, Miura S, Ezaki O, Ogawa Y.
Title Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner.
Journal FEBS Lett
Abstract Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues.
Volume 581(1)
Pages 91-6
Published 2007-1-9
DOI 10.1016/j.febslet.2006.12.002
PII S0014-5793(06)01432-3
PMID 17182038
MeSH Adipocytes / metabolism* Adipocytes / pathology Adipose Tissue / metabolism* Adipose Tissue / pathology Alleles* Alternative Splicing Animals Cell Line Cell Size Exons Gene Expression Regulation* Genomic Imprinting* Humans Mice Mice, Obese Neoplasms / genetics Neoplasms / metabolism Neoplasms / pathology Obesity / genetics Obesity / metabolism* Obesity / pathology Protein Isoforms / biosynthesis Protein Isoforms / genetics Proteins / genetics Proteins / metabolism* Transgenes
IF 3.057
Times Cited 36
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Human and Animal Cells RAW 264(RCB0535)