論文 - 詳細
| RRC ID | 42857 |
|---|---|
| 著者 | Shoji H, Oguchi S, Shinohara K, Shimizu T, Yamashiro Y. |
| タイトル | Effects of iron-unsaturated human lactoferrin on hydrogen peroxide-induced oxidative damage in intestinal epithelial cells. |
| ジャーナル | Pediatr Res |
| Abstract |
Human milk (HM) contains various bioactive antioxidants. Lactoferrin (Lf) has been assumed to be one of the major antioxidants in HM. We examined the antioxidative properties of iron-unsaturated human Lf (apo-hLf, the major form of Lf in HM) in two intestinal epithelial cell lines: (1) An intestinal epithelial cell line (IEC-6) were preincubated for 24 h with either 50 microg/mL of apo-hLf, iron-saturated human Lf (holo-hLf), iron-unsaturated bovine transferrin (apo-bTf), or 800 ng/mL of the iron-chelating compound deferoxamine (DFX), followed by hydrogen peroxide (H2O2) challenge to induce oxidative stress. Survival rates were significantly higher in the cells preincubated with apo-hLf and DFX than those preincubated with holo-hLf. (2) Caco-2 cells were preincubated with or without apo-hLf for 24 h, followed by an H2O2 challenge. Intracellular oxidative stress was assessed by a fluorescent probe, 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Fluorescent intensity of cell images and cell homogenates was significantly lower in the cells preincubated with apo-hLF than those preincubated without apo-hLF. Our study indicates that apo-hLf alleviates H2O2-induced oxidative damage in intestinal cells due to the iron-chelating capacity. Therefore, Lf in HM may act as an antioxidant in the gastrointestinal tract (GIT). |
| 巻・号 | 61(1) |
| ページ | 89-92 |
| 公開日 | 2007-1-1 |
| DOI | 10.1203/01.pdr.0000250198.22735.20 |
| PII | 00006450-200701000-00018 |
| PMID | 17211147 |
| MeSH | Animals Cattle Cell Line Dose-Response Relationship, Drug Epithelial Cells / drug effects Epithelial Cells / metabolism Humans Hydrogen Peroxide / pharmacology* Intestinal Mucosa / drug effects Intestinal Mucosa / metabolism* Iron / metabolism* Lactoferrin / physiology* Milk, Human / physiology Oxidants / pharmacology* Oxidative Stress / physiology* Rats |
| IF | 2.747 |
| 引用数 | 43 |
| WOS 分野 | PEDIATRICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | IEC 6(RCB0993) |