Reference - Detail
| RRC ID | 42859 |
|---|---|
| Author | Narukawa M, Suzuki N, Takayama T, Yamashita Y, Otsuka K, Ito K. |
| Title | Enamel matrix derivative stimulates osteogenesis- and chondrogenesis-related transcription factors in C3H10T1/2 cells. |
| Journal | Acta Biochim Biophys Sin (Shanghai) |
| Abstract |
Our purpose was to determine how enamel matrix derivative (EMD) affects the expression of osteogenesis- and chondrogenesis-related transcription factors in undifferentiated mesenchymal cells. C3H10T1/2 cell line, a typical pluripotential mesenchymal cell line, was cultured with or without EMD for up to 7 d. Expression of mRNAs encoding osteogenesis- and chondrogenesis-related transcription factors (Runx2, Osterix, AJ18, Dlx5, Msx2, Sox5, Sox9 and Zfp60) was measured using real-time polymerase chain reaction. Runx2 and Sox9 protein expression and the presence of bone morphogenetic protein (BMP)-6-like molecules in EMD were determined by Western blotting. EMD substantially increased mRNA levels of osteogenesis- and chondrogenesis-related transcription factors. EMD also induced Runx2 and Sox9 protein expression. Western blotting analysis of EMD using anti-BMP-6 antibody revealed immunoreactive bands corresponding to about 14 kDa and 60 kDa. These results suggest that EMD stimulates osteogenesis- and chondrogenesis-related transcription factors, and these activities may be mediated, at least in part, by BMP-6 in EMD. |
| Volume | 39(1) |
| Pages | 1-7 |
| Published | 2007-1-1 |
| DOI | 10.1111/j.1745-7270.2007.00250.x |
| PMID | 17213952 |
| MeSH | Animals Bone Morphogenetic Protein 6 Bone Morphogenetic Proteins / analysis Cells, Cultured Chondrogenesis / genetics* Collagen Type II / genetics Dental Enamel Proteins / pharmacology* Gene Expression Regulation / drug effects* Mice Osteocalcin / genetics Osteogenesis / genetics* RNA, Messenger / analysis |
| IF | 2.836 |
| Times Cited | 13 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | |