| RRC ID |
42861
|
| 著者 |
Hedlund E, Pruszak J, Ferree A, Viñuela A, Hong S, Isacson O, Kim KS.
|
| タイトル |
Selection of embryonic stem cell-derived enhanced green fluorescent protein-positive dopamine neurons using the tyrosine hydroxylase promoter is confounded by reporter gene expression in immature cell populations.
|
| ジャーナル |
Stem Cells
|
| Abstract |
Transplantation of mouse embryonic stem (mES) cells can restore function in Parkinson disease models, but can generate teratomas. Purification of dopamine neurons derived from embryonic stem cells by fluorescence-activated cell sorting (FACS) could provide a functional cell population for transplantation while eliminating the risk of teratoma formation. Here we used the tyrosine hydroxylase (TH) promoter to drive enhanced green fluorescent protein (eGFP) expression in mES cells. First, we evaluated 2.5-kilobase (kb) and 9-kb TH promoter fragments and showed that clones generated using the 9-kb fragment produced significantly more eGFP+/TH+ neurons. We selected the 9-kb TH clone with the highest eGFP/TH overlap for further differentiation, FACS, and transplantation experiments. Grafts contained large numbers of eGFP+ dopamine neurons of an appropriate phenotype. However, there were also numerous eGFP+ cells that did not express TH and did not have a neuronal morphology. In addition, we found cells in the grafts representing all three germ layers. Based on these findings, we examined the expression of stem cell markers in our eGFP+ population. We found that a majority of eGFP+ cells were stage-specific embryonic antigen-positive (SSEA-1+) and that the genetically engineered clones contained more SSEA-1+ cells after differentiation than the original D3 mES cells. By negative selection of SSEA-1, we could isolate a neuronal eGFP+ population of high purity. These results illustrate the complexity of using genetic selection to purify mES cell-derived dopamine neurons and provide a comprehensive analysis of cell selection strategies based on tyrosine hydroxylase expression. Disclosure of potential conflicts of interest is found at the end of this article.
|
| 巻・号 |
25(5)
|
| ページ |
1126-35
|
| 公開日 |
2007-5-1
|
| DOI |
10.1634/stemcells.2006-0540
|
| PII |
2006-0540
|
| PMID |
17234989
|
| PMC |
PMC2614084
|
| MeSH |
Animals
Cell Differentiation
Cell Proliferation
Dopamine / metabolism*
Embryonic Stem Cells / cytology*
Flow Cytometry
Gene Expression
Genes, Reporter*
Green Fluorescent Proteins / metabolism*
Lewis X Antigen / metabolism
Mice
Mice, Inbred C57BL
Neurons / cytology*
Promoter Regions, Genetic / genetics*
Rats
Rats, Sprague-Dawley
Stem Cell Transplantation
Transplantation, Heterologous
Tyrosine 3-Monooxygenase / genetics*
|
| IF |
6.022
|
| 引用数 |
53
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
ONCOLOGY
HEMATOLOGY
CELL & TISSUE ENGINEERING
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127) |
