RRC ID 42866
Author Shimada S, Nakamura M, Tanaka Y, Tsutsumi K, Katano M, Masuko K, Yudoh K, Koizuka I, Kato T.
Title Crosslinking of the CD69 molecule enhances S100A9 production in activated neutrophils.
Journal Microbiol Immunol
Abstract Expression of CD69 on neutrophils and generation of anti-CD69 autoantibodies in patients with rheumatoid arthritis (RA) have been reported. Thus natural ligands for CD69 not yet identified and/or the anti-CD69 autoantibodies possibly affect neutrophils by evoking CD69 signaling, which may further affect joint-composing cells in RA. However, the effect of the CD69 signaling in neutrophils remains largely unclear. To elucidate the issue, we tried to identify proteins affected by the crosslinking of CD69 on neutrophils using a proteomic approach. Specifically, CD69 on granulocyte-macrophage colony stimulating factor (GM-CSF)-activated neutrophils was crosslinked by anti-CD69 monoclonal antibodies, and then intracellular proteins were detected using 2-dimensional electrophoresis and further identified by mass spectrometry and subsequent protein database searching. As a result, we successfully identified multiple proteins that increased their production by the CD69 signaling. Among the proteins, we focused on one of the up-regulated proteins, S100A9 calcium binding protein (S100A9), and investigated proteome changes brought by a recombinant S100A9 in a human synovial sarcoma cell line (SW982), a human chondrosarcoma cell line (OUMS-27), and a human T leukemia cell line (Jurkat). This revealed that the recombinant S100A9 altered proteomes of SW982 and OUMS-27, and to a lesser extent, that of the Jurkat cells. Further, S100A9 induced IL-1beta production from neutrophils and the SW982 cells. These data suggest that unidentified natural ligands for CD69 and/or the anti-CD69 autoantibodies possibly affect joint-composing cell types through the increased production of S100A9 in neutrophils, providing a new insight into functions of CD69 on neutrophils in RA.
Volume 51(1)
Pages 87-98
Published 2007-1-1
DOI 10.1111/j.1348-0421.2007.tb03878.x
PII JST.JSTAGE/mandi/51.87
PMID 17237603
MeSH Adult Antibodies, Monoclonal / immunology Antigens, CD / immunology Antigens, CD / metabolism* Antigens, Differentiation, T-Lymphocyte / immunology Antigens, Differentiation, T-Lymphocyte / metabolism* Autoantibodies / immunology Calgranulin B / biosynthesis* Cell Line, Tumor Cells, Cultured Electrophoresis, Gel, Two-Dimensional Female Gene Expression Regulation Granulocyte-Macrophage Colony-Stimulating Factor / immunology Humans Jurkat Cells Lectins, C-Type Ligands Mass Spectrometry Neutrophils / immunology* Neutrophils / metabolism* Proteome / analysis
IF 1.566
Times Cited 12
Human and Animal Cells