| RRC ID |
42876
|
| 著者 |
Christakos S, Dhawan P, Peng X, Obukhov AG, Nowycky MC, Benn BS, Zhong Y, Liu Y, Shen Q.
|
| タイトル |
New insights into the function and regulation of vitamin D target proteins.
|
| ジャーナル |
J Steroid Biochem Mol Biol
|
| Abstract |
Calbindin-D(28k) has been reported to be a facilitator of calcium diffusion and to protect against apoptotic cell death. Most recently, we found that the presence of calbindin-D(28k) results in reduced calcium influx through voltage-dependent L-type Ca(2+) channels and enhanced sensitivity of the channels to calcium dependent inactivation. Co-immunoprecipitation and GST pull down assays indicate that calbindin-D(28k) interacts with the C-terminus of the L-type calcium channel alpha(1c) subunit (Ca(v)1.2). This is the first report of the binding of calbindin to a calcium channel and provides new insight concerning mechanisms by which calbindin acts to modulate intracellular calcium. Besides calbindin, another major target of 1,25(OH)(2)D(3) is 24(OH)ase, which is involved in the catabolism of 1,25(OH)(2)D(3). We reported that C/EBPbeta is a major transcriptional activator of 24(OH)ase that cooperates with CBP/p300 in regulating VDR mediated 24(OH)ase transcription. Recently, we found, in addition to p160 coactivators, that SWI/SNF complexes (that facilitate transcription by remodeling chromatin using the energy of ATP hydrolysis) are also involved in VDR mediated 24(OH)ase transcription and functionally cooperate with C/EBPbeta in regulating 24(OH)ase. These findings define novel mechanisms that may be of fundamental importance in understanding how 1,25(OH)(2)D(3) mediates its multiple biological effects.
|
| 巻・号 |
103(3-5)
|
| ページ |
405-10
|
| 公開日 |
2007-3-1
|
| DOI |
10.1016/j.jsbmb.2006.12.079
|
| PII |
S0960-0760(06)00445-6
|
| PMID |
17257825
|
| PMC |
PMC2859311
|
| MeSH |
Animals
Calbindins
Calcium / metabolism
Cell Line
Cholestanetriol 26-Monooxygenase / metabolism
Chromatin / genetics
Electrophysiology
Ion Channel Gating
Mutation / genetics
Patch-Clamp Techniques
Rats
Receptors, Calcitriol / metabolism
S100 Calcium Binding Protein G / metabolism
Vitamin D / metabolism*
|
| IF |
3.813
|
| 引用数 |
17
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
