| RRC ID |
42884
|
| 著者 |
Yamashiro T, Zheng L, Shitaku Y, Saito M, Tsubakimoto T, Takada K, Takano-Yamamoto T, Thesleff I.
|
| タイトル |
Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis.
|
| ジャーナル |
Differentiation
|
| Abstract |
We have explored the role of Wnt signaling in dentinogenesis of mouse molar teeth. We found that Wnt10a was specifically associated with the differentiation of odontoblasts and that it showed striking colocalization with dentin sialophosphoprotein (Dspp) expression in secretory odontoblasts. Dspp is a tooth specific non-collagenous matrix protein and regulates dentin mineralization. Transient overexpression of Wnt10 in C3H10T1/2, a pluripotent fibroblast cell line induced Dspp mRNA. Interestingly, this induction occurred only when transfected cells were cultured on Matrigel basement membrane extracts. These findings indicated that Wnt10a is an upstream regulatory molecule for Dspp expression, and that cell-matrix interaction is essential for induction of Dspp expression. Furthermore, Wnt10a was specifically expressed in the epithelial signaling centers regulating tooth development, the primary and secondary enamel knots. The spatial and temporal distribution of Wnt10a mRNA demonstrated that the expression shifts from the secondary enamel knots, to the underlying preodontoblasts in the tips of future cusps. The expression patterns and overexpression studies together indicate that Wnt10a is a key molecule for dentinogenesis and that it is associated with the cell-matrix interactions regulating odontoblast differentiation. We conclude that Wnt10a may link the differentiation of odontoblasts and cusp morphogenesis.
|
| 巻・号 |
75(5)
|
| ページ |
452-62
|
| 公開日 |
2007-6-1
|
| DOI |
10.1111/j.1432-0436.2006.00150.x
|
| PII |
S0301-4681(09)60138-9
|
| PMID |
17286598
|
| MeSH |
Animals
Blotting, Western
Cell Differentiation*
Cell Membrane
Collagen / metabolism
Drug Combinations
Epithelial Cells / metabolism
Extracellular Matrix Proteins
In Situ Hybridization
Laminin / metabolism
Mice
Mice, Inbred C3H
Morphogenesis*
Nerve Tissue Proteins / physiology*
Odontoblasts / cytology*
Odontoblasts / metabolism
Phosphoproteins
Pluripotent Stem Cells / metabolism
Protein Precursors / genetics*
Protein Precursors / metabolism
Proteoglycans / metabolism
RNA, Messenger / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sialoglycoproteins
Signal Transduction
Tooth / embryology*
Tooth / metabolism
Wnt Proteins / physiology*
|
| IF |
2.392
|
| 引用数 |
90
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
